Nitrogen isotopic fractionation during a simulated diatom spring bloom: importance of N-starvation in controlling fractionation

	Nitrogen isotopic fractionation during a simulated diatom spring bloom: importance of N-starvation in controlling fractionation
	Waser, NA ; Yu, ZM; Yin, KD ; Nielsen, B ; Harrison, PJ ; Turpin, DH ; Calvert, SE
	1999
发表期刊	MARINE ECOLOGY PROGRESS SERIES
ISSN	0171-8630
卷号	179 页码:291-296
文章类型	Article
摘要	N isotope fractionation (epsilon) was first determined during ambient NO3- depletion in a simulated diatom spring bloom. After 48 h of N-starvation, NH4+ was resupplied to the diatoms in small pulses to simulate grazer-produced N and then epsilon was determined. Large variations in epsilon values were observed: from 2.0-3.6 to 14-0 parts per thousand during NO3- and NH4+ uptake, respectively. This is the first study reporting an epsilon value as low as 0 to 2 parts per thousand for NH4+ uptake and we suggest that greater N demand after N-starvation may have drastically reduced NH3 efflux out of the cells. Thus the N status of the phytoplankton and not the ambient NH4+ concentration may be the important factor controlling epsilon, because, when N-starvation increased, epsilon values for NH4+ uptake decreased within 30 h. This study may thus have important implications for interpreting the delta(15)N of particulate N in nutrient-depleted regimes in temperate coastal oceans.; N isotope fractionation (epsilon) was first determined during ambient NO3- depletion in a simulated diatom spring bloom. After 48 h of N-starvation, NH4+ was resupplied to the diatoms in small pulses to simulate grazer-produced N and then epsilon was determined. Large variations in epsilon values were observed: from 2.0-3.6 to 14-0 parts per thousand during NO3- and NH4+ uptake, respectively. This is the first study reporting an epsilon value as low as 0 to 2 parts per thousand for NH4+ uptake and we suggest that greater N demand after N-starvation may have drastically reduced NH3 efflux out of the cells. Thus the N status of the phytoplankton and not the ambient NH4+ concentration may be the important factor controlling epsilon, because, when N-starvation increased, epsilon values for NH4+ uptake decreased within 30 h. This study may thus have important implications for interpreting the delta(15)N of particulate N in nutrient-depleted regimes in temperate coastal oceans.
关键词	n Isotope Fractionation N-15/n-14 Nitrate Uptake Ammonium Uptake Diatoms Spring Bloom
学科领域	Ecology ; Marine & Freshwater Biology ; Oceanography
收录类别	SCI
语种	英语
WOS记录号	WOS:000080261400025
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2848
专题	海洋生态与环境科学重点实验室
作者单位	1.Univ British Columbia, Dept Earth & Ocean Sci, Vancouver, BC V6T 1Z4, Canada 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 3.Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada
推荐引用方式 GB/T 7714	Waser, NA,Yu, ZM,Yin, KD,et al. Nitrogen isotopic fractionation during a simulated diatom spring bloom: importance of N-starvation in controlling fractionation[J]. MARINE ECOLOGY PROGRESS SERIES,1999,179:291-296.
APA	Waser, NA.,Yu, ZM.,Yin, KD.,Nielsen, B.,Harrison, PJ.,...&Calvert, SE.(1999).Nitrogen isotopic fractionation during a simulated diatom spring bloom: importance of N-starvation in controlling fractionation.MARINE ECOLOGY PROGRESS SERIES,179,291-296.
MLA	Waser, NA,et al."Nitrogen isotopic fractionation during a simulated diatom spring bloom: importance of N-starvation in controlling fractionation".MARINE ECOLOGY PROGRESS SERIES 179(1999):291-296.

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