Study on cytocompatibility of carboxymethyl-chitosan membranes to skin fibroblasts

	Study on cytocompatibility of carboxymethyl-chitosan membranes to skin fibroblasts
	Zheng, L ; Chen, XG ; Liu, WS ; Han, XT ; Yan, XJ
	2003-04-01
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN	1000-3282
卷号	30 期号:2 页码:314-320
文章类型	Article
摘要	Chitosan and carboxymethl-chitosan (CM-chitosan) membranes with different molecular mass were prepared by a casting method. The cytocompatibility of two kinds of polysaccharide membranes to skin fibroblasts that cultured in vitro were studied. The methods were to culture the cells in soaking fluid of membranes and to culture the cells on the membranes directly. The results showed that the soaking fluid had no toxicity to fibroblasts and the biological security of lower molecular mass membranes were better than higher molecular mass membranes, and CM-chitosan membranes were better than chitosan membranes. In addition, the growth of fibroblasts on chitosan membranes was inhibited and the cells would fall off from chitosan membranes after a period of culture. However, the cells adhered and expanded well on CM-chitosan membranes. All these demonstrated that cytocompatibility of CM-chitosan membranes to skin fibroblasts was better than chitosan membranes.; Chitosan and carboxymethl-chitosan (CM-chitosan) membranes with different molecular mass were prepared by a casting method. The cytocompatibility of two kinds of polysaccharide membranes to skin fibroblasts that cultured in vitro were studied. The methods were to culture the cells in soaking fluid of membranes and to culture the cells on the membranes directly. The results showed that the soaking fluid had no toxicity to fibroblasts and the biological security of lower molecular mass membranes were better than higher molecular mass membranes, and CM-chitosan membranes were better than chitosan membranes. In addition, the growth of fibroblasts on chitosan membranes was inhibited and the cells would fall off from chitosan membranes after a period of culture. However, the cells adhered and expanded well on CM-chitosan membranes. All these demonstrated that cytocompatibility of CM-chitosan membranes to skin fibroblasts was better than chitosan membranes.
关键词	Chitosan Carboxymethl-chitosan (Cm-chitosan) Membrane Skin Fibroblast Cytocompatibility
学科领域	Biochemistry & Molecular Biology ; Biophysics
收录类别	SCI
语种	英语
WOS记录号	WOS:000182764700028
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2808
专题	海洋生态与环境科学重点实验室
作者单位	1.Ocean Univ Qingdao, Life Sci Coll, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zheng, L,Chen, XG,Liu, WS,et al. Study on cytocompatibility of carboxymethyl-chitosan membranes to skin fibroblasts[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(2):314-320.
APA	Zheng, L,Chen, XG,Liu, WS,Han, XT,&Yan, XJ.(2003).Study on cytocompatibility of carboxymethyl-chitosan membranes to skin fibroblasts.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,30(2),314-320.
MLA	Zheng, L,et al."Study on cytocompatibility of carboxymethyl-chitosan membranes to skin fibroblasts".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 30.2(2003):314-320.

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