Modulatory effects of ammonia-N on the immune system of Penaeus japonicus to virulence of white spot syndrome virus

doi:10.1016/j.aquaculture.2004.08.020

	Modulatory effects of ammonia-N on the immune system of Penaeus japonicus to virulence of white spot syndrome virus
	Jiang, GJ ; Yu, RC ; Zhou, MJ
	2004-11-26
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	241 期号:1-4 页码:61-75
文章类型	Article
摘要	To study response to white spot syndrome virus (WSSV) under ammonia stress, Penaeus japonicus were exposed to 5 mg l(-1) ammonia-N and challenged orally with WSSV (NW). Controls consisted of an ammonia-N-exposed control group (N), a WSSV-challenged positive control group (W), and an untreated control group (control). Immune parameters measured were total haemocyte count (THC), haemocyte phagocytosis, plasma protein content and haemolymph enzymatic activities for prophenoloxidase (proPO), alkaline phosphatase (ALP), and nitric oxide synthase (NOS). THC and plasma protein had downward trends with time in all treatment groups (NW, N, and W) in contrast to the untreated control group (control). The percentage phagocytosis, NOS activity, and ALP and proPO activity of W and NW decreased initially then increased from 6 to 78 h (except for NOS and ALP, from 6 to 54 h) before declining thereafter until the end of the experiment. Compared with untreated controls (control), there was a downward trend for all measured parameters in the treatment groups (N, NW, and W), but the degree was W > NW > N. WSSV was detected at 78 h postchallenge in both W and NW. In conclusion, 5 mg l(-1) ammonia-N reduced the immunocompetence of P japonicus and may have decreased the virulence of WSSV (C) 2004 Elsevier B.V. All rights reserved.; To study response to white spot syndrome virus (WSSV) under ammonia stress, Penaeus japonicus were exposed to 5 mg l(-1) ammonia-N and challenged orally with WSSV (NW). Controls consisted of an ammonia-N-exposed control group (N), a WSSV-challenged positive control group (W), and an untreated control group (control). Immune parameters measured were total haemocyte count (THC), haemocyte phagocytosis, plasma protein content and haemolymph enzymatic activities for prophenoloxidase (proPO), alkaline phosphatase (ALP), and nitric oxide synthase (NOS). THC and plasma protein had downward trends with time in all treatment groups (NW, N, and W) in contrast to the untreated control group (control). The percentage phagocytosis, NOS activity, and ALP and proPO activity of W and NW decreased initially then increased from 6 to 78 h (except for NOS and ALP, from 6 to 54 h) before declining thereafter until the end of the experiment. Compared with untreated controls (control), there was a downward trend for all measured parameters in the treatment groups (N, NW, and W), but the degree was W > NW > N. WSSV was detected at 78 h postchallenge in both W and NW. In conclusion, 5 mg l(-1) ammonia-N reduced the immunocompetence of P japonicus and may have decreased the virulence of WSSV (C) 2004 Elsevier B.V. All rights reserved.
关键词	Penaeus Japonicus White Spot Syndrome Virus (Wssv) Ammonia-n Immune Parameter
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/j.aquaculture.2004.08.020
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000225070700006
引用统计	被引频次：69[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2728
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Jiang, GJ,Yu, RC,Zhou, MJ. Modulatory effects of ammonia-N on the immune system of Penaeus japonicus to virulence of white spot syndrome virus[J]. AQUACULTURE,2004,241(1-4):61-75.
APA	Jiang, GJ,Yu, RC,&Zhou, MJ.(2004).Modulatory effects of ammonia-N on the immune system of Penaeus japonicus to virulence of white spot syndrome virus.AQUACULTURE,241(1-4),61-75.
MLA	Jiang, GJ,et al."Modulatory effects of ammonia-N on the immune system of Penaeus japonicus to virulence of white spot syndrome virus".AQUACULTURE 241.1-4(2004):61-75.

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