Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Evaluation of potential internal references for quantitative real-time RT-PCR normalization of gene expression in red drum (Sciaenops ocellatus) | |
Sun, Bo-guang; Hu, Yong-hua | |
2015-06-01 | |
发表期刊 | FISH PHYSIOLOGY AND BIOCHEMISTRY |
卷号 | 41期号:3页码:695-704 |
文章类型 | Article |
摘要 | Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) has been used extensively for studying gene expression in diverse organisms including fish. In this study, with an aim to identify reliable reference genes for qRT-PCR in red drum (Sciaenops ocellatus), an economic fish species, we determined the expression stability of seven housekeeping genes in healthy and bacterium-infected red drum. Each of the selected candidate genes was amplified by qRT-PCR from the brain, gill, heart, intestine, kidney, liver, muscle, and spleen of red drum infected with or without a bacterial pathogen for 12 and 48 h. The mRNA levels of the genes were analyzed with the geNorm and NormFinder algorithms. The results showed that in the absence of bacterial infection, translation initiation factor 3, NADH dehydrogenase 1, and QM-like protein may be used together as internal references across the eight examined tissues. Bacterial infection caused variations in the rankings of the most stable genes in a tissue-dependent manner. For all tissues, two genes sufficed for reliable normalization at both 12 and 48 h post-infection. However, the optimal gene pairs differed among tissues and, for four of the examined eight tissues, between infection points. These results indicate that when studying gene expression in red drum under conditions of bacterial infection, the optimal reference genes should be selected on the basis of tissue type and, for accurate normalization, infection stage. |
关键词 | Housekeeping Gene Expression Stability Reference Gene Sciaenops Ocellatus |
DOI | 10.1007/s10695-015-0039-8 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000354619300011 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/26965 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
第一作者单位 | 实验海洋生物学重点实验室 |
推荐引用方式 GB/T 7714 | Sun, Bo-guang,Hu, Yong-hua. Evaluation of potential internal references for quantitative real-time RT-PCR normalization of gene expression in red drum (Sciaenops ocellatus)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(3):695-704. |
APA | Sun, Bo-guang,&Hu, Yong-hua.(2015).Evaluation of potential internal references for quantitative real-time RT-PCR normalization of gene expression in red drum (Sciaenops ocellatus).FISH PHYSIOLOGY AND BIOCHEMISTRY,41(3),695-704. |
MLA | Sun, Bo-guang,et al."Evaluation of potential internal references for quantitative real-time RT-PCR normalization of gene expression in red drum (Sciaenops ocellatus)".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.3(2015):695-704. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Evaluation of potent(1693KB) | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 |
个性服务 |
推荐该条目 |
保存到收藏夹 |
查看访问统计 |
导出为Endnote文件 |
谷歌学术 |
谷歌学术中相似的文章 |
[Sun, Bo-guang]的文章 |
[Hu, Yong-hua]的文章 |
百度学术 |
百度学术中相似的文章 |
[Sun, Bo-guang]的文章 |
[Hu, Yong-hua]的文章 |
必应学术 |
必应学术中相似的文章 |
[Sun, Bo-guang]的文章 |
[Hu, Yong-hua]的文章 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论