Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu

doi:10.1007/s00343-009-9141-z

	Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu
	Zhao Weihong 1; Wang Jiangtao 2; Chen Meimei 1
	2009-08-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	27 期号:3 页码:564-569
文章类型	Article
摘要	Filtration and cross-flow ultrafiltration techniques were used to separate culture media of Prorocentrum donghaiense at the exponential growth, stationary and decline stages into < 0.45 mu m filtrate, 100 kDa-0.45 mu m, 10-100 kDa and 1-10 kDa retentate and < 1 kDa ultrafiltrate fractions. The fluorescence. properties of different molecular weights of dissolved organic matter (DOM) were measured by excitation-emission matrix spectra. Protein-like and humic-like fluorophores were observed in the DOM produced by P. donghaiense. The central positions of protein-like fluorophores showed a red shift with prolonged growth duration, shifting from tyrosine-like properties at the exponential growth stage to tryptophan-like properties at the stationary and decline stages. The excitation wavelengths of protein-like fluorophores exhibited some change in the exponential growth and stationary stages with increased molecular size, but showed little change in the decline stage. However, the emission wavelengths in the decline stage exhibited a blue shift. Very distinct C type and A type peaks in humic-like fluorophores were observed. With a prolonged culture time, the intensities of both of the peaks became strong and the excitation wavelengths of peak A showed a red shift, while the A:C ratios fell. More than 94% of fluorescent DOM was in the lower than 1 kDa molecular weight fraction.; Filtration and cross-flow ultrafiltration techniques were used to separate culture media of Prorocentrum donghaiense at the exponential growth, stationary and decline stages into < 0.45 mu m filtrate, 100 kDa-0.45 mu m, 10-100 kDa and 1-10 kDa retentate and < 1 kDa ultrafiltrate fractions. The fluorescence. properties of different molecular weights of dissolved organic matter (DOM) were measured by excitation-emission matrix spectra. Protein-like and humic-like fluorophores were observed in the DOM produced by P. donghaiense. The central positions of protein-like fluorophores showed a red shift with prolonged growth duration, shifting from tyrosine-like properties at the exponential growth stage to tryptophan-like properties at the stationary and decline stages. The excitation wavelengths of protein-like fluorophores exhibited some change in the exponential growth and stationary stages with increased molecular size, but showed little change in the decline stage. However, the emission wavelengths in the decline stage exhibited a blue shift. Very distinct C type and A type peaks in humic-like fluorophores were observed. With a prolonged culture time, the intensities of both of the peaks became strong and the excitation wavelengths of peak A showed a red shift, while the A:C ratios fell. More than 94% of fluorescent DOM was in the lower than 1 kDa molecular weight fraction.
关键词	Dissolved Organic Matter Fluorescence Excitation-emission Matrix Spectrum Prorocentrum Donghaiense Lu
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-009-9141-z
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000270742000020
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2676
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhao Weihong,Wang Jiangtao,Chen Meimei. Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):564-569.
APA	Zhao Weihong,Wang Jiangtao,&Chen Meimei.(2009).Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),564-569.
MLA	Zhao Weihong,et al."Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):564-569.

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