Isolation and identification of bacteria associated with the surfaces of several algal species

doi:10.1007/s00343-009-9165-4

	Isolation and identification of bacteria associated with the surfaces of several algal species
	Wang Zifeng 1,2; Xiao Tian 1; Pang Shaojun 1; Liu Min 1; Yue Haidong 1
	2009-08-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	27 期号:3 页码:487-492
文章类型	Article
摘要	We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses. Twelve strains of bacteria were obtained from the surfaces of the following four species of algae: Gracilaria textorii, Ulva pertusa, Laminaria japonica, and Polysiphonia urceolata. The isolated strains of bacteria can be divided into two groups: Halomonas and Vibrio, in physiology, biochemical characteristics and 16S rDNA sequence analyses. The phylogenetic tree constructed based on 16S rDNA sequences of the isolates shows four obvious clusters, Halomonas venusta, Vibrio tasmaniensis, Vibrio lentus, and Vibrio splendidus. Isolates from the surface of P. urceolata are more abundant and diverse, of which strains P9 and P28 have a 16S rDNA sequence very similar (97.5%-99.8%) to that of V. splendidus. On the contrary, the isolates from the surfaces of G textorii, U. pertusa and L. japonica are quite simple and distribute on different branches of the phylogenetic tree. In overall, the results of this study indicate that the genetic relationships among the isolates are quite close and display a certain level of host species specificity, and alga-associated bacteria species are algal species specific.; We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses. Twelve strains of bacteria were obtained from the surfaces of the following four species of algae: Gracilaria textorii, Ulva pertusa, Laminaria japonica, and Polysiphonia urceolata. The isolated strains of bacteria can be divided into two groups: Halomonas and Vibrio, in physiology, biochemical characteristics and 16S rDNA sequence analyses. The phylogenetic tree constructed based on 16S rDNA sequences of the isolates shows four obvious clusters, Halomonas venusta, Vibrio tasmaniensis, Vibrio lentus, and Vibrio splendidus. Isolates from the surface of P. urceolata are more abundant and diverse, of which strains P9 and P28 have a 16S rDNA sequence very similar (97.5%-99.8%) to that of V. splendidus. On the contrary, the isolates from the surfaces of G textorii, U. pertusa and L. japonica are quite simple and distribute on different branches of the phylogenetic tree. In overall, the results of this study indicate that the genetic relationships among the isolates are quite close and display a certain level of host species specificity, and alga-associated bacteria species are algal species specific.
关键词	Epiphytic Bacteria Algae 16s Rrna Gene Phylogeny
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-009-9165-4
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000270742000009
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2642
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang Zifeng,Xiao Tian,Pang Shaojun,et al. Isolation and identification of bacteria associated with the surfaces of several algal species[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):487-492.
APA	Wang Zifeng,Xiao Tian,Pang Shaojun,Liu Min,&Yue Haidong.(2009).Isolation and identification of bacteria associated with the surfaces of several algal species.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),487-492.
MLA	Wang Zifeng,et al."Isolation and identification of bacteria associated with the surfaces of several algal species".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):487-492.

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