海水培养对叶用甜菜形态和生理特性的影响

IOCAS-IR > 海洋环流与波动重点实验室

	海水培养对叶用甜菜形态和生理特性的影响
	孙兰菊
学位类型	博士
	2000
学位授予单位	中国科学院海洋研究所
学位授予地点	中国科学院海洋研究所
学位专业	海洋生物学
关键词	海水培养形态和生理特性渗透压脯氨酸可溶性糖电解质渗漏率蛋白质
摘要	本文以含有不同浓度海水的50％Hoagland培养液进行叶用甜菜（Beta vulgaris var. cicla L.）的无土培养，并研究了海水培养条件下叶用甜菜多种形态和生理特性的变化情况。结果表明，在电导率相当于22％海水的培养液中生长时，叶用甜菜植株形态和显微结构均无明显变化。在电导率相当于41％海水的培养液中生长时，叶用甜菜仍能保持存活和生长，显微观察也未发现死亡细胞，但植株形态和生理特性发生某些变化：（1）植株形态变化：植株叶面积大大减少，叶片增厚，栅栏组织细胞膨大，根毛变短；（2）植株干重降低，细胞渗透压提高，含水量增加（3）游离脯氨酸的含量比对照增加137％；（4）可溶性糖的含量基本未发生变化；（5）细胞膜电解质渗漏率增大，细胞内离子含量增加；（6）多肽成分变化：24.4KD2和7.0KD多肽的量明显增加；29.1 KD和29.5 KD多肽的表达量则有所减少等。结果表明至少海水培养液电导率相当于22％海水时，叶用甜菜能够正常的生和长，当培养液中盐度过高时，叶用甜菜的形态和生理特性发生某些变经，但仍能生存和长生。同时这些变化也说明叶用甜菜的耐盐性涉及多种生理生化过程，是由体内多种结构和功能协同进行的。
其他摘要	Beta vulgaris var. cicla L. seedlings were treated with different concentrations of seawater, and some physiological and biochemistry changes were studied 20 days after treatment. Beta vulgaris var. cicla L. seedlings can survive and grow when treated with 16.7% and 33.3% seawater, but T-2 seedlings showed some changes in physiological and biochemistry characters. In seawater-free and 33.3% seawater-containing media the width of leaves was decreased by 45%; the length was decreased by 53%; and the thickness was increased by 168%. When treated with 33.3% seawater, the amount of stomata per cm on leaves decreased, at the same time, palisade cells and spongy parenchyma cells were expanded and root hair became shorter and fewer. The dry weight of T-1 and T-2 seedlings were decreased by 31% and 55%, with the water concentration increased by 4.6% and 6.9%. In T-1 and T-2 seedling, the osmotic pressure of the cells was increased by 25% and 75%. In leaves of T-1 and T-2 seedling, the concentration of Proline was increased by 72% and 137%, but the concentration of soluble sugars remained approximately the same. The leakage rate of electrolyte of the leaves of T-1 and T-2 was increased by 83% and 130%, which indicated that the function of cell membrane was effected by the treatment. The protein patterns were determined in leaves and roots of T-1 and T-2. The results showed that the amount of 24.4kD and 27.0kD protein were increased and the amount of 29.1kD and 29.5kD protein were decreased. The changes of physiological and biochemistry characters and Beta vulgarish var. cicla L. seedlings treated with seawater showed that when grow in seawater-containing media, the seedlings change in these characters to adapt to environment.
页数	63
语种	中文
文献类型	学位论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/263
专题	海洋环流与波动重点实验室
推荐引用方式 GB/T 7714	孙兰菊. 海水培养对叶用甜菜形态和生理特性的影响[D]. 中国科学院海洋研究所. 中国科学院海洋研究所,2000.

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