IOCAS-IR  > 海洋生态与环境科学重点实验室
Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater
Liu Xiu-Juan1,2; Yu Zhi-Ming1; Song Xiu-Xian1; Cao Xi-Hua1
2009-05-01
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume37Issue:5Pages:643-647
SubtypeArticle
AbstractA suitable method for the pretreutment of dissolved nitrate samples in seawaters for nitrogen isotopic analysis was established. First, the seawater samples were processed by removing nitrite and amonium. Then Devard's alloy was added in sample for conversion of dissolved nitrate to ammonium. The sample was distilled, and then the ammonium condensate was collected with zeolite. after distillation, the collected condensate was filtered and prepared for determining nitropic values. Some tests of the method were conducted. The distillation condition, the influence of salinity on nitrogen isotopic analysis, absorption of ammonium onto zeolite and an improved method on a large volume of seawater were discussed in this study. The results showed that the distillation step had an average recovery of (104.9 +/- 4.2) % (n = 6) when distillating every 300 mL aliquot of the sample under a strong alkaline condition with 0.5 g devard's alloy and a distillation time of 30 min. The nitrogen isotopic fractionation decreased markedly when salinity was increased from 0% to 0.5%; further increase(1% - 3.5%) showed little effect. The adsorption rate of ammonium onto zeolite had a high yield of (95.96 +/- 1.08) % (n = 6) in average. An improved collection method was used to process a large volume of seawater with several distillations, and had good effect on analysis. The method had been applied to analyze water samples collected from Changjiang estuary. The analytical results indicate that the method is suitable for delta(15)N analysis of dissolved nitrate in seawaters. The present method could provide valuable information about the source and cycle mechanism of dissolved nitrogen in estuary waters.; A suitable method for the pretreutment of dissolved nitrate samples in seawaters for nitrogen isotopic analysis was established. First, the seawater samples were processed by removing nitrite and amonium. Then Devard's alloy was added in sample for conversion of dissolved nitrate to ammonium. The sample was distilled, and then the ammonium condensate was collected with zeolite. after distillation, the collected condensate was filtered and prepared for determining nitropic values. Some tests of the method were conducted. The distillation condition, the influence of salinity on nitrogen isotopic analysis, absorption of ammonium onto zeolite and an improved method on a large volume of seawater were discussed in this study. The results showed that the distillation step had an average recovery of (104.9 +/- 4.2) % (n = 6) when distillating every 300 mL aliquot of the sample under a strong alkaline condition with 0.5 g devard's alloy and a distillation time of 30 min. The nitrogen isotopic fractionation decreased markedly when salinity was increased from 0% to 0.5%; further increase(1% - 3.5%) showed little effect. The adsorption rate of ammonium onto zeolite had a high yield of (95.96 +/- 1.08) % (n = 6) in average. An improved collection method was used to process a large volume of seawater with several distillations, and had good effect on analysis. The method had been applied to analyze water samples collected from Changjiang estuary. The analytical results indicate that the method is suitable for delta N-15 analysis of dissolved nitrate in seawaters. The present method could provide valuable information about the source and cycle mechanism of dissolved nitrogen in estuary waters.
KeywordSeawater System Dissolved Nitrate Stable Nitrogen Isotopic Analysis Preparation Method
Subject AreaChemistry, Analytical
Indexed BySCI
Language英语
WOS IDWOS:000266385500003
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/2618
Collection海洋生态与环境科学重点实验室
文献信息中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu Xiu-Juan,Yu Zhi-Ming,Song Xiu-Xian,et al. Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(5):643-647.
APA Liu Xiu-Juan,Yu Zhi-Ming,Song Xiu-Xian,&Cao Xi-Hua.(2009).Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(5),643-647.
MLA Liu Xiu-Juan,et al."Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.5(2009):643-647.
Files in This Item:
File Name/Size DocType Version Access License
liu-Pretreatment Met(316KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu Xiu-Juan]'s Articles
[Yu Zhi-Ming]'s Articles
[Song Xiu-Xian]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu Xiu-Juan]'s Articles
[Yu Zhi-Ming]'s Articles
[Song Xiu-Xian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu Xiu-Juan]'s Articles
[Yu Zhi-Ming]'s Articles
[Song Xiu-Xian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: liu-Pretreatment Method for Nitrogen Isotopic.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.