中国科学院海洋研究所机构知识库
Advanced  
IOCAS-IR  > 海洋生态与环境科学重点实验室  > 期刊论文
题名: A bioaugmentation failure caused by phage infection and weak biofilm formation ability
作者: Fu Songzhe1,2; Fan Hongxia1,2; Liu Shuangjiang1; Liu, Ying(刘鹰)3; Liu Zhipei1
刊名: JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
出版日期: 2009
卷号: 21, 期号:8, 页码:1153-1161
关键词: marine culture ; water circulation ; ammonia removal ; Lutimonas sp H10 ; nitrification breakdown ; fluorescent in situ hybridization (FISH)
学科分类: Environmental Sciences
DOI: 10.1016/S1001-0742(08)62396-7
文章类型: Article
英文摘要: Two biological aerated filters (BAF) were setup for ammonia removal treatment of the circulation water in a marine aquaculture. One of the BAFs was bioaugmented with a heterotrophic nitrifying bacterium, Lutimonas sp. H10, where the ammonia removal was not improved and the massive inoculation was even followed by a nitrification breakdown from day 9 to 18. The nitrification was remained stable in control BAF operated under the same conditions. Fluorescent in situ hybridization (FISH) with rRNA-targeted probes and cultivable method revealed that Lutimonas sp. H10 almost disappeared from the bioaugomented BAF within 3 d, and this was mainly due to the infection of a specific phage as revealed by flask experiment, plaque assay and transmission electron observation. Analyses of 16S rRNA gene libraries showed that bacterial groups from two reactors evolved differently and an overgrowth of protozoa was observed in the bioaugmented BAR Therefore, phage infection and poor biofilm forming ability of the inoculated strain are the main reasons for bioaugmentation failure. In addition, gazing by protozoa of the bacteria might be the reason for the nitrification breakdown in bioaugmented BAF during day 9-18.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000269139100020
中文摘要: Two biological aerated filters (BAF) were setup for ammonia removal treatment of the circulation water in a marine aquaculture. One of the BAFs was bioaugmented with a heterotrophic nitrifying bacterium, Lutimonas sp. H10, where the ammonia removal was not improved and the massive inoculation was even followed by a nitrification breakdown from day 9 to 18. The nitrification was remained stable in control BAF operated under the same conditions. Fluorescent in situ hybridization (FISH) with rRNA-targeted probes and cultivable method revealed that Lutimonas sp. H10 almost disappeared from the bioaugomented BAF within 3 d, and this was mainly due to the infection of a specific phage as revealed by flask experiment, plaque assay and transmission electron observation. Analyses of 16S rRNA gene libraries showed that bacterial groups from two reactors evolved differently and an overgrowth of protozoa was observed in the bioaugmented BAR Therefore, phage infection and poor biofilm forming ability of the inoculated strain are the main reasons for bioaugmentation failure. In addition, gazing by protozoa of the bacteria might be the reason for the nitrification breakdown in bioaugmented BAF during day 9-18.
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.qdio.ac.cn/handle/337002/2605
Appears in Collections:海洋生物技术研发中心_期刊论文
海洋生态与环境科学重点实验室_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
fu-A bioaugmentation failure caused.pdf(377KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266701, Peoples R China

Recommended Citation:
Fu Songzhe; Fan Hongxia; Liu Shuangjiang; Liu Ying; Liu Zhipei.A bioaugmentation failure caused by phage infection and weak biofilm formation ability,JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2009,21(8):1153-1161
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Fu Songzhe]'s Articles
[Fan Hongxia]'s Articles
[Liu Shuangjiang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Fu Songzhe]‘s Articles
[Fan Hongxia]‘s Articles
[Liu Shuangjiang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: fu-A bioaugmentation failure caused.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院海洋研究所 - Feedback
Powered by CSpace