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A bioaugmentation failure caused by phage infection and weak biofilm formation ability
Fu Songzhe1,2; Fan Hongxia1,2; Liu Shuangjiang1; Liu Ying3; Liu Zhipei1
2009
发表期刊JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
ISSN1001-0742
卷号21期号:8页码:1153-1161
文章类型Article
摘要Two biological aerated filters (BAF) were setup for ammonia removal treatment of the circulation water in a marine aquaculture. One of the BAFs was bioaugmented with a heterotrophic nitrifying bacterium, Lutimonas sp. H10, where the ammonia removal was not improved and the massive inoculation was even followed by a nitrification breakdown from day 9 to 18. The nitrification was remained stable in control BAF operated under the same conditions. Fluorescent in situ hybridization (FISH) with rRNA-targeted probes and cultivable method revealed that Lutimonas sp. H10 almost disappeared from the bioaugomented BAF within 3 d, and this was mainly due to the infection of a specific phage as revealed by flask experiment, plaque assay and transmission electron observation. Analyses of 16S rRNA gene libraries showed that bacterial groups from two reactors evolved differently and an overgrowth of protozoa was observed in the bioaugmented BAR Therefore, phage infection and poor biofilm forming ability of the inoculated strain are the main reasons for bioaugmentation failure. In addition, gazing by protozoa of the bacteria might be the reason for the nitrification breakdown in bioaugmented BAF during day 9-18.; Two biological aerated filters (BAF) were setup for ammonia removal treatment of the circulation water in a marine aquaculture. One of the BAFs was bioaugmented with a heterotrophic nitrifying bacterium, Lutimonas sp. H10, where the ammonia removal was not improved and the massive inoculation was even followed by a nitrification breakdown from day 9 to 18. The nitrification was remained stable in control BAF operated under the same conditions. Fluorescent in situ hybridization (FISH) with rRNA-targeted probes and cultivable method revealed that Lutimonas sp. H10 almost disappeared from the bioaugomented BAF within 3 d, and this was mainly due to the infection of a specific phage as revealed by flask experiment, plaque assay and transmission electron observation. Analyses of 16S rRNA gene libraries showed that bacterial groups from two reactors evolved differently and an overgrowth of protozoa was observed in the bioaugmented BAR Therefore, phage infection and poor biofilm forming ability of the inoculated strain are the main reasons for bioaugmentation failure. In addition, gazing by protozoa of the bacteria might be the reason for the nitrification breakdown in bioaugmented BAF during day 9-18.
关键词Marine Culture Water Circulation Ammonia Removal Lutimonas Sp H10 Nitrification Breakdown Fluorescent In Situ Hybridization (Fish)
学科领域Environmental Sciences
DOI10.1016/S1001-0742(08)62396-7
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000269139100020
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/2604
专题海洋生态与环境科学重点实验室
海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266701, Peoples R China
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Fu Songzhe,Fan Hongxia,Liu Shuangjiang,et al. A bioaugmentation failure caused by phage infection and weak biofilm formation ability[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2009,21(8):1153-1161.
APA Fu Songzhe,Fan Hongxia,Liu Shuangjiang,Liu Ying,&Liu Zhipei.(2009).A bioaugmentation failure caused by phage infection and weak biofilm formation ability.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,21(8),1153-1161.
MLA Fu Songzhe,et al."A bioaugmentation failure caused by phage infection and weak biofilm formation ability".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 21.8(2009):1153-1161.
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