Edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway

doi:10.1016/j.fsi.2014.12.037

IOCAS-IR > 实验海洋生物学重点实验室

	Edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway
	Li, Mo-fei1,2 ; Sun, Li 1; Li, Jun 3
	2015-04-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	43 期号:2 页码:325-329
文章类型	Article
摘要	Edwardsiella tarda is a Gram-negative bacterium with a broad host range that includes a wide variety of farmed fish as well as humans. E. tarda has long been known to be able to survive in host serum, but the relevant mechanism is unclear. In this study, we investigated the fundamental question, i.e. whether E. tarda activated serum complement or not. We found that (i) when incubated with flounder serum, E. tarda exhibited a high survival rate (87.6%), which was slightly but significantly reduced in the presence of Mg2+; (ii) E. tarda-incubated serum possessed strong hemolytic activity and bactericidal activity, (iii) compared to the serum incubated with a complement-sensitive laboratory Escherichia coli strain, E. tarda-incubated serum exhibited much less chemotactic activity, (iv) in contrast to the serum incubated with live E. tarda, the serum incubated with heat-inactivated E. tarda exhibited no apparent hemolytic capacity. Taken together, these results indicate for the first time that E. tarda circumvents serum attack by preventing, to a large extent, complement activation via the alternative pathway, and that heatlabile surface structures likely play an essential role in the complement evasion of E. tarda. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Serum Resistance Immune Evasion Virulence Bacteria
DOI	10.1016/j.fsi.2014.12.037
收录类别	SCI
语种	英语
WOS记录号	WOS:000352923700004
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25474
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI 49783 USA
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Mo-fei,Sun, Li,Li, Jun. Edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(2):325-329.
APA	Li, Mo-fei,Sun, Li,&Li, Jun.(2015).Edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway.FISH & SHELLFISH IMMUNOLOGY,43(2),325-329.
MLA	Li, Mo-fei,et al."Edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway".FISH & SHELLFISH IMMUNOLOGY 43.2(2015):325-329.

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