Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Differential gene expression in Staphylococcus aureus exposed to Orange II and Sudan III azo dyes | |
| Pan, Hongmiao1,2; Xu, Joshua3; Kweon, Oh-Gew1; Zou, Wen3; Feng, Jinhui1; He, Gui-Xin4; Cerniglia, Carl E.1; Chen, Huizhong1 | |
| 2015-05-01 | |
| 发表期刊 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
 |
| 卷号 | 42期号:5页码:745-757 |
| 文章类型 | Article |
| 摘要 | We previously demonstrated the effects of azo dyes and their reduction metabolites on bacterial cell growth and cell viability. In this report, the effects of Orange II and Sudan III on gene expression profiling in Staphylococcus aureus ATCC BAA 1556 were analyzed using microarray and quantitative RT-PCR technology. Upon exposure to 6 mu g/ml Orange II for 18 h, 21 genes were found to be differently expressed. Among them, 8 and 13 genes were up- and down-regulated, respectively. Most proteins encoded by these differentially expressed genes involve stress response caused by drug metabolism, oxidation, and alkaline shock indicating that S. aureus could adapt to Orange II exposure through a balance between up and down regulated gene expression. Whereas, after exposure to 6 mu g/ml Sudan III for 18 h, 57 genes were differentially expressed. In which, 51 genes were up-regulated and 6 were down-regulated. Most proteins encoded by these differentially expressed genes involve in cell wall/membrane biogenesis and biosynthesis, nutrient uptake, transport and metabolite, and stress response, suggesting that Sudan III damages the bacterial cell wall or/and membrane due to binding of the dye. Further analysis indicated that all differentially expressed genes encoded membrane proteins were up-regulated and most of them serve as transporters. The result suggested that these genes might contribute to survival, persistence and growth in the presence of Sudan III. Only one gene msrA, which plays an important role in oxidative stress resistance, was found to be down-regulated after exposure to both Orange II and Sudan III. The present results suggested that both these two azo dyes can cause stress in S. aureus and the response of the bacterium to the stress is mainly related to characteristics of the azo dyes. |
| 关键词 | Staphylococcus Aureus Orange Ii Sudan Iii Microarray Gene Expression |
| DOI | 10.1007/s10295-015-1599-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352860600009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/25473 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.US FDA, Div Microbiol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.US FDA, Div Bioinformat & Biostat, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA 4.Univ Massachusetts, Dept Clin Lab & Nutr Sci, Lowell, MA 01854 USA |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Pan, Hongmiao,Xu, Joshua,Kweon, Oh-Gew,et al. Differential gene expression in Staphylococcus aureus exposed to Orange II and Sudan III azo dyes[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(5):745-757. |
| APA | Pan, Hongmiao.,Xu, Joshua.,Kweon, Oh-Gew.,Zou, Wen.,Feng, Jinhui.,...&Chen, Huizhong.(2015).Differential gene expression in Staphylococcus aureus exposed to Orange II and Sudan III azo dyes.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(5),745-757. |
| MLA | Pan, Hongmiao,et al."Differential gene expression in Staphylococcus aureus exposed to Orange II and Sudan III azo dyes".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.5(2015):745-757. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Differential gene ex（416KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论