Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection

doi:10.1016/j.vetmic.2015.01.020

IOCAS-IR > 实验海洋生物学重点实验室

	Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection
	Sun, Yuan-yuan1,2 ; Sun, Li 1
	2015-04-17
发表期刊	VETERINARY MICROBIOLOGY
卷号	176 期号:3-4 页码:309-320
文章类型	Article
摘要	For pathogenic bacteria, the ability to acquire iron is vital to survival in the host. In consequence, many genes involved in iron acquisition are associated with bacterial virulence. Pseudomonas fluorescens is a bacterial pathogen to a variety of farmed fish. However, the global regulatory function of iron in pathogenic P. fluorescens is essentially unknown. In this study, in order to identify proteins affected by iron condition at the expression level, we performed proteomic analysis to compare the global protein profiles of P. fluorescens strain TSS, a fish pathogen, cultured under iron-replete and iron-deplete conditions. Twenty-two differentially expressed proteins were identified, most of which were confirmed to be regulated by iron at the mRNA level. To investigate their potential involvement in virulence, the genes encoding four of the 22 proteins, i.e. HemO (heme oxygenase), PspB (serine protease), Sod (superoxide dismutase), and TfeR (TonB-dependent outermembrane ferric enterobactin receptor), were knocked out, and the pathogenicity of the mutants was examined in a model of turbot (Scophthalmus maximus). The results showed that compared to the wild type, the hemO, pspB, and tfeR knockouts were significantly impaired in the ability to survive in host serum, to invade host tissues, and to cause host mortality. Immunization of turbot with recombinant TfeR (rTfeR) and PspB induced production of specific serum antibodies and significant protections against lethal TSS challenge. Further analysis showed that rTfeR antibodies recognized and bound to TSS, and that treatment of TSS with rTfeR antibodies significantly impaired the infectivity of TSS to fish cells. Taken together, these results indicate for the first time that in pathogenic P. fluorescens, iron affects the expression of a large number of proteins including those that are involved in host infection. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Pseudomonas Fluorescens Iron Proteomic Virulence Immunoprotection
DOI	10.1016/j.vetmic.2015.01.020
收录类别	SCI
语种	英语
WOS记录号	WOS:000352047600012
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25461
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Sun, Yuan-yuan,Sun, Li. Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection[J]. VETERINARY MICROBIOLOGY,2015,176(3-4):309-320.
APA	Sun, Yuan-yuan,&Sun, Li.(2015).Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection.VETERINARY MICROBIOLOGY,176(3-4),309-320.
MLA	Sun, Yuan-yuan,et al."Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection".VETERINARY MICROBIOLOGY 176.3-4(2015):309-320.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Pseudomonas fluoresc（1359KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览