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Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus)
Lin, Zhengmei1; Zhu, Xiangping1; You, Feng2; Wu, Zhihao2; Cao, Yuanshui3
2015-05-01
Source PublicationTHERIOGENOLOGY
Volume83Issue:8Pages:1310-1320
SubtypeArticle
AbstractSperm genetic material of olive flounder (Paralichthys olivaceus) was inactivated by ultraviolet irradiation. The nuclear phase changes during early embryonic development of diploid, haploid, and mitogynogenetic diploid induced by hydrostatic pressure treatment were observed under fluorescent microscope with 4',6-diamidino-2-phenylindole staining. The parameters of hydrostatic pressure treatment were 600 kg/cm(2) for 6 minutes at prometaphase stage. The data showed that developmental timing sequence of diploid and haploid fertilized eggs was similar. The cell cycle was about 48 minutes, including interphase (about 21 minutes), prophase (about 3 minutes), prometaphase (about 6 minutes), metaphase (about 6 minutes), anaphase (around 9 minutes), and telophase (about 3 minutes). After entering the fertilized egg, ultraviolet-inactivated sperm formed a male pronucleus and became a dense chromatin body in the cytoplasm. Dense chromatin body did not participate in nuclear division and unchanged all the time. For hydrostatic pressure-treated embryos, the first nuclear division and cytokinesis after treatment proceeded normally after about 15 minutes recovery. During the second mitosis, having undergone interphase, prophase, and prometaphase stage, chromosomes began to slowly spread around and scattered in the cell but not entered into metaphase and anaphase. The second nuclear division and cytokinesis was inhibited. The occurrence frequency of developmentally delayed embryos also showed that the second cleavage of about 80% treated eggs was inhibited. The inhibition of the second cleavage resulted to chromosome set doubling. So chromosome set doubling for mitogynogenetic flounder diploid induced by hydrostatic pressure treatment, performed at prometaphase stage, was mainly due to inhibition of the second mitosis rather than the first one. (C) 2015 Elsevier Inc. All rights reserved.
KeywordOlive Flounder (Paralichthys Olivaceus) Mitogynogenetic Diploid Fluorescent Microscopic Observation Hydrostatic Pressure
DOI10.1016/j.theriogenology.2015.01.020
Indexed BySCI
Language英语
WOS IDWOS:000352175300008
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/25451
Collection实验海洋生物学重点实验室
Affiliation1.Qingdao Agr Univ, Anim Sci & Technol Dept, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
3.Shenghang Aquat Sci & Technol Co Ltd, Weihai, Peoples R China
Recommended Citation
GB/T 7714
Lin, Zhengmei,Zhu, Xiangping,You, Feng,et al. Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus)[J]. THERIOGENOLOGY,2015,83(8):1310-1320.
APA Lin, Zhengmei,Zhu, Xiangping,You, Feng,Wu, Zhihao,&Cao, Yuanshui.(2015).Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus).THERIOGENOLOGY,83(8),1310-1320.
MLA Lin, Zhengmei,et al."Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus)".THERIOGENOLOGY 83.8(2015):1310-1320.
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