IOCAS-IR  > 实验海洋生物学重点实验室
Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus)
Lin, Zhengmei1; Zhu, Xiangping1; You, Feng2; Wu, Zhihao2; Cao, Yuanshui3
2015-05-01
发表期刊THERIOGENOLOGY
卷号83期号:8页码:1310-1320
文章类型Article
摘要Sperm genetic material of olive flounder (Paralichthys olivaceus) was inactivated by ultraviolet irradiation. The nuclear phase changes during early embryonic development of diploid, haploid, and mitogynogenetic diploid induced by hydrostatic pressure treatment were observed under fluorescent microscope with 4',6-diamidino-2-phenylindole staining. The parameters of hydrostatic pressure treatment were 600 kg/cm(2) for 6 minutes at prometaphase stage. The data showed that developmental timing sequence of diploid and haploid fertilized eggs was similar. The cell cycle was about 48 minutes, including interphase (about 21 minutes), prophase (about 3 minutes), prometaphase (about 6 minutes), metaphase (about 6 minutes), anaphase (around 9 minutes), and telophase (about 3 minutes). After entering the fertilized egg, ultraviolet-inactivated sperm formed a male pronucleus and became a dense chromatin body in the cytoplasm. Dense chromatin body did not participate in nuclear division and unchanged all the time. For hydrostatic pressure-treated embryos, the first nuclear division and cytokinesis after treatment proceeded normally after about 15 minutes recovery. During the second mitosis, having undergone interphase, prophase, and prometaphase stage, chromosomes began to slowly spread around and scattered in the cell but not entered into metaphase and anaphase. The second nuclear division and cytokinesis was inhibited. The occurrence frequency of developmentally delayed embryos also showed that the second cleavage of about 80% treated eggs was inhibited. The inhibition of the second cleavage resulted to chromosome set doubling. So chromosome set doubling for mitogynogenetic flounder diploid induced by hydrostatic pressure treatment, performed at prometaphase stage, was mainly due to inhibition of the second mitosis rather than the first one. (C) 2015 Elsevier Inc. All rights reserved.
关键词Olive Flounder (Paralichthys Olivaceus) Mitogynogenetic Diploid Fluorescent Microscopic Observation Hydrostatic Pressure
DOI10.1016/j.theriogenology.2015.01.020
收录类别SCI
语种英语
WOS记录号WOS:000352175300008
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/25451
专题实验海洋生物学重点实验室
作者单位1.Qingdao Agr Univ, Anim Sci & Technol Dept, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
3.Shenghang Aquat Sci & Technol Co Ltd, Weihai, Peoples R China
推荐引用方式
GB/T 7714
Lin, Zhengmei,Zhu, Xiangping,You, Feng,et al. Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus)[J]. THERIOGENOLOGY,2015,83(8):1310-1320.
APA Lin, Zhengmei,Zhu, Xiangping,You, Feng,Wu, Zhihao,&Cao, Yuanshui.(2015).Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus).THERIOGENOLOGY,83(8),1310-1320.
MLA Lin, Zhengmei,et al."Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus)".THERIOGENOLOGY 83.8(2015):1310-1320.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Nuclei fluorescence (2375KB)期刊论文出版稿限制开放ODC PDDL浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lin, Zhengmei]的文章
[Zhu, Xiangping]的文章
[You, Feng]的文章
百度学术
百度学术中相似的文章
[Lin, Zhengmei]的文章
[Zhu, Xiangping]的文章
[You, Feng]的文章
必应学术
必应学术中相似的文章
[Lin, Zhengmei]的文章
[Zhu, Xiangping]的文章
[You, Feng]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。