Microbial Fe (III) reduction and hydrogen production by a transposon-mutagenized strain of Pantoea agglomerans BH18

doi:10.1016/j.energy.2015.01.014

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	Microbial Fe (III) reduction and hydrogen production by a transposon-mutagenized strain of Pantoea agglomerans BH18
	Liu, Hongyan 1; Wang, Guangce 1,2
	2015-03-01
发表期刊	ENERGY
卷号	81 页码:691-695
文章类型	Article
摘要	Based on the transposon-mutagenized library of Pantoea agglomerans BH18, mutant screens were conducted to obtain the strain with the highest Fe (III) reduction and hydrogen production. Of these transposon-mutagenized mutants, the mutant strain TB230 was screened for high Fe (III)-reducing efficiency and hydrogen production. The PCR amplification and kanamycin resistance selection results indicated that the transposon insertion of the mutant strain TB230 was stable. Hydrogen production of the mutant strain TB230 was (2.21 +/- 0.34) mol H-2/mol glucose, which increased hydrogen production by over 40% compared with that of the wild type strain. The accumulation concentration of Fe (II) in the medium of the mutant strain TB230 with Fe (OH)(3) as the sole electron acceptor was (7.39 +/- 0.49) mmol/l, which was approximately 3-fold greater than that of the wild type strain. The mutant strain TB230 showed high Fe (III)-reducing activity and hydrogen production by adopting glucose and pyruvate as the carbon source. In addition, the mutant strain TB230 was capable of Fe (III) reduction and hydrogen production under-fresh or marine conditions. This result indicates that the mutant strain with high microbial Fe (III) reduction and hydrogen production is beneficial for the improvement of anaerobic performance. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Transposon-mutagenized Library Mutant Screen Hydrogen Production Fe (Iii) Reduction Fe (Ii) Concentration
DOI	10.1016/j.energy.2015.01.014
收录类别	SCI
语种	英语
WOS记录号	WOS:000351248200066
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25442
专题	实验海洋生物学重点实验室
作者单位	1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hongyan,Wang, Guangce. Microbial Fe (III) reduction and hydrogen production by a transposon-mutagenized strain of Pantoea agglomerans BH18[J]. ENERGY,2015,81:691-695.
APA	Liu, Hongyan,&Wang, Guangce.(2015).Microbial Fe (III) reduction and hydrogen production by a transposon-mutagenized strain of Pantoea agglomerans BH18.ENERGY,81,691-695.
MLA	Liu, Hongyan,et al."Microbial Fe (III) reduction and hydrogen production by a transposon-mutagenized strain of Pantoea agglomerans BH18".ENERGY 81(2015):691-695.

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Microbial Fe (III) r（730KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览