实验海洋生物学重点实验室知识库

QQ客服 官方微博 反馈留言

	Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21
	Li Jiang1; Sha Yujie2
	2015-03-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号	33期号:2页码:319-327
文章类型	Article
摘要	An agar-degrading bacterium, designated as Pseudoalteromonas sp. NJ21, was isolated from an Antarctic sediment sample. The agarase gene aga1161 from Pseudoalteromonas sp. NJ21 consisting of a 2 382-bp coding region was cloned. The gene encodes a 793-amino acids protein and was found to possess characteristic features of the Glyco_hydro_42 family. The recombinant agarase (rAga1161) was overexpressed in Escherichia coli and purified as a fusion protein. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were 30-40A degrees C and 8.0, respectively. rAga1161 was found to maintain as much as 80% of its maximum activity at 10A degrees C, which is typical of a coldadapted enzyme. The pattern of agar hydrolysis demonstrated that the enzyme is an beta-agarase, producing neoagarobiose (NA2) as the final main product. Furthermore, this work is the first proof of an agarolytic activity in Antarctic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.
关键词	Antarctic Bacterium Pseudoalteromonas Agarase Expression Enzymatic Characterization
DOI	10.1007/s00343-015-4072-3
收录类别	SCI
语种	英语
WOS记录号	WOS:000351156800004
引用统计	被引频次：4[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25403
专题	实验海洋生物学重点实验室
作者单位	1.SOA, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li Jiang,Sha Yujie. Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(2):319-327.
APA	Li Jiang,&Sha Yujie.(2015).Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(2),319-327.
MLA	Li Jiang,et al."Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.2(2015):319-327.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Expression and enzym（1958KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览

个性服务

推荐该条目

保存到收藏夹

查看访问统计

导出为Endnote文件

谷歌学术

谷歌学术中相似的文章

[Li Jiang]的文章

[Sha Yujie]的文章

百度学术

百度学术中相似的文章

[Li Jiang]的文章

[Sha Yujie]的文章

必应学术

必应学术中相似的文章

[Li Jiang]的文章

[Sha Yujie]的文章

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。