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	Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)
	Liu, Q. H.1,2; Ma, D. Y.1; Xu, S. H.1; Xiao, Z. Z.1; Xiao, Y. S.1; Song, Z. C.3; Li, J.1,2
	2015-03-01
发表期刊	THERIOGENOLOGY
卷号	83期号:4页码:703-710
文章类型	Article
摘要	The present study established an efficient technology for summer flounder (Paralichthys dentatus) sperm cryopreservation, and successfully applied the cryopreserved sperm into interspecific hybridization with olive flounder (P olivaceus). The best motility of postthaw sperm (78.00 +/- 4.70% and 76.60 +/- 7.90%), fertilization rates (95.70 +/- 3.60% and 79.40 +/- 5.20%), and hatching rates (93.10 +/- 4.00% and 77.20 +/- 2.90%) were achieved when using propylene glycol or DMSO as cryoprotectant. Furthermore, we have successfully improved the cryopreservation method from using 2-mL cryotube to 5-mL cryotube, and the dilution ratio has been increased to 4:1. By this method, the cryopreservation efficiency has been improved to 30 times of that with routine method. Computer-assisted sperm motion analysis showed the freezing thawing process decreased the sperm speed but did not significantly change the sperm movement pattern, and the progressive linear motion still was the dominant movement pattern. The ultrastructural analysis showed 50% to 60% of the spermatozoa had normal morphology, 20% to 30% were slightly damaged, such as swelling or rupture of head, midpiece, and tail region, and 10% to 20% were severely damaged. In the artificial hybridization experiment, high fertilization rates and hatching rates were achieved when using 15% DMSO (95.7 +/- 3.6% and 79.4 +/- 5.2%, respectively) and 15% propylene glycol (93.1 +/- 4.0% and 77.2 +/- 2.9%, respectively), with no significant difference in comparison with control (94.7 +/- 2.6% and 72.5 +/- 6.5%, respectively). In addition, we found the embryos and larvae from postthaw sperm of P dentatus developed and grew normally. The results of the present study further validated the safety of the cryopreserved sperm in breeding production by assessing the fertilization capacity, embryo development, and larval growth. (C) 2015 Elsevier Inc. All rights reserved.
关键词	Summer Flounder Sperm Cryopreservation Interspecific Hybridization Olive Flounder
DOI	10.1016/j.theriogenology.2014.11.004
收录类别	SCI
语种	英语
WOS记录号	WOS:000349512800032
引用统计	被引频次：26[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25376
专题	海洋生物技术研发中心 实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China 3.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Q. H.,Ma, D. Y.,Xu, S. H.,et al. Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)[J]. THERIOGENOLOGY,2015,83(4):703-710.
APA	Liu, Q. H..,Ma, D. Y..,Xu, S. H..,Xiao, Z. Z..,Xiao, Y. S..,...&Li, J..(2015).Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus).THERIOGENOLOGY,83(4),703-710.
MLA	Liu, Q. H.,et al."Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)".THERIOGENOLOGY 83.4(2015):703-710.

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