Immune effects of R848: Evidences that suggest an essential role of TLR7/8-induced, Myd88-and NF-kappa B-dependent signaling in the antiviral immunity of Japanese flounder (Paralichthys olivaceus)

doi:10.1016/j.dci.2014.11.018

IOCAS-IR > 实验海洋生物学重点实验室

	Immune effects of R848: Evidences that suggest an essential role of TLR7/8-induced, Myd88-and NF-kappa B-dependent signaling in the antiviral immunity of Japanese flounder (Paralichthys olivaceus)
	Zhou, Zhi-xia; Sun, Li
	2015-03-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	49 期号:1 页码:113-120
文章类型	Article
摘要	The imidazoquinoline compound R848 is a specific agonist of toll-like receptor (TLR) 7/TLR8 that has been used as an immunostimulant in humans against viral diseases. Although R848-induced immune response has been reported in teleost fish, the relevant mechanism is not clear. In this study, we investigated the antiviral potential and the signaling pathway of R848 in a model of Japanese flounder (Paralichthys olivaceus). We found that R848 was able to inhibit the replication of megalocytivirus, stimulated the proliferation of peripheral blood leukocytes (PBL), enhanced the expression of immune genes, and reduced apoptosis of PBL. When endosomal acidification was blocked by chloroquine (CQ), R848-mediated antiviral activity and immune response were significantly reduced. Likewise, inhibition of Myd88 activation markedly impaired the pro-proliferation and anti-apoptosis effect of R848. Cellular study showed that cultured founder cells treated with R848 exhibited augmented NF-kappa B activity, which, however, was dramatically reduced in the presence of CQand Myd88 inhibitor. Furthermore, when NF-kappa B was inactivated, the effect of R848 on cell proliferation and apoptosis was significantly decreased. Taken together, these results indicate that R848 is an immunostimulant with antiviral property in a teleost species, and that the immune response of R848 is mediated by, most likely, TLR7/TLR8 signaling pathway, in which Myd88 and NK--kappa B play an essential role. (C) 2014 Elsevier Ltd. All rights reserved.
关键词	R848 Toll-like Receptor Myd88 Nk-kappa b Teleost Antiviral Immunity
DOI	10.1016/j.dci.2014.11.018
收录类别	SCI
语种	英语
WOS记录号	WOS:000348889800013
引用统计	被引频次：45[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25368
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhou, Zhi-xia,Sun, Li. Immune effects of R848: Evidences that suggest an essential role of TLR7/8-induced, Myd88-and NF-kappa B-dependent signaling in the antiviral immunity of Japanese flounder (Paralichthys olivaceus)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,49(1):113-120.
APA	Zhou, Zhi-xia,&Sun, Li.(2015).Immune effects of R848: Evidences that suggest an essential role of TLR7/8-induced, Myd88-and NF-kappa B-dependent signaling in the antiviral immunity of Japanese flounder (Paralichthys olivaceus).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,49(1),113-120.
MLA	Zhou, Zhi-xia,et al."Immune effects of R848: Evidences that suggest an essential role of TLR7/8-induced, Myd88-and NF-kappa B-dependent signaling in the antiviral immunity of Japanese flounder (Paralichthys olivaceus)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 49.1(2015):113-120.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Evidences that sugge（2030KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览