Purification and Characterization of Chitinases from Ridgetail White Prawn Exopalaemon carinicauda

doi:10.3390/molecules20021955

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	Purification and Characterization of Chitinases from Ridgetail White Prawn Exopalaemon carinicauda
	Wang, Jing 1,2; Zhang, Jiquan 1; Song, Fengge1,2 ; Gui, Tianshu1,2 ; Xiang, Jianhai1
	2015-02-01
发表期刊	MOLECULES
卷号	20 期号:2 页码:1955-1967
文章类型	Article
摘要	In this paper, we purified two native chitinases from the hepatopancreas of the ridgetail white prawn Exopalaemon carinicauda by using ion-exchange resin chromatography (IEC) and gel filtration. These two chitinases, named EcChi1 and EcChi2, were identified by chitinolytic activity assay and LC-ESI-MS/MS. Their apparent molecular weights were 44 kDa and 65 kDa as determined by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The specific activity of EcChi1 and EcChi2 was 1305.97 U center dot mg(-1) and 28.69 U center dot mg(-1). The optimal temperature and pH of EcChi1 were 37 degrees C and pH 4.0, respectively. Co2+, Fe3+, Zn2+, Cd2+, and Cu2+ had an obvious promoting effect upon chitinase activity of EcChi1. For colloidal chitin, the K-m and V-max values of EcChi1 were 2.09 mg center dot mL(-1) and 31.15 U center dot mL(-1)center dot h(-1).
DOI	10.3390/molecules20021955
收录类别	SCI
语种	英语
WOS记录号	WOS:000350748200011
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25354
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Jing,Zhang, Jiquan,Song, Fengge,et al. Purification and Characterization of Chitinases from Ridgetail White Prawn Exopalaemon carinicauda[J]. MOLECULES,2015,20(2):1955-1967.
APA	Wang, Jing,Zhang, Jiquan,Song, Fengge,Gui, Tianshu,&Xiang, Jianhai.(2015).Purification and Characterization of Chitinases from Ridgetail White Prawn Exopalaemon carinicauda.MOLECULES,20(2),1955-1967.
MLA	Wang, Jing,et al."Purification and Characterization of Chitinases from Ridgetail White Prawn Exopalaemon carinicauda".MOLECULES 20.2(2015):1955-1967.

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Purification and Cha（7722KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览