Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The polymorphisms in the promoter of HSP90 gene and their association with heat tolerance of bay scallop | |
Yang, Chuanyan1; Wang, Lingling1; Liu, Conghui2 ; Zhou, Zhi1; Zhao, Xin1; Song, Linsheng1
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| 2015-03-01 | |
| 发表期刊 | CELL STRESS & CHAPERONES
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| 卷号 | 20期号:2页码:297-308 |
| 文章类型 | Article |
| 摘要 | The heat shock protein 90 (HSP90) is a highly abundant and ubiquitous molecular chaperone which plays essential roles in many cellular processes. In the present study, the messenger RNA (mRNA) expressions of HSP90 after acute heat stress were investigated in two bay scallop populations (Argopecten irradians irradians and Argopecten irradians concentricus). The heat-resistant scallop A. i. concentricus, which is distributed in Zhanjiang, China, exhibited significantly higher induction of HSP90 compared with that of the heat-sensitive scallop A. i. irradians, which is distributed in Qinhuangdao, China. The promoter sequence of HSP90 gene from bay scallop (AiHSP90) was cloned, and the polymorphisms within this region were investigated by sequencing to analyze their association with heat tolerance. A total of six single nucleotide polymorphisms (SNPs), including -1167 T-C, -1023 A-C, -799 C-T, -774 A-G, -686 C-T, and -682 A-C, were identified in the amplified promoter region, and most of them affected the putative transcription factor binding sites except for locus -1167. All the six SNP sites were found to be associated with heat tolerance after Hardy-Weinberg equilibrium (HWE) and association analysis. Moreover, haplotypes CACACC and TCTATC were also found to be associated with heat tolerance based on the result of linkage disequilibrium and association analysis. The results provided insights into the molecular mechanisms underlying the thermal adaptation of different congener endemic bay scallops, which suggested that the increased heat tolerance of A. i. concentricus (compared with A. i. irradians) was associated with the higher expression of AiHSP90. Meanwhile, the six genotypes (-1167 TT, -1023 CC, -799 TT, -774 GG, -686 CC, and -682 AA) and two haplotypes (CACACC and TCTATC) could be used as potential markers for scallop selection breeding with higher heat tolerance. |
| 关键词 | Argopecten Irradians Heat Shock Protein 90 Promoter Single Nucleotide Polymorphism Heat Tolerance |
| DOI | 10.1007/s12192-014-0546-z |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349907600011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/25349 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, Chuanyan,Wang, Lingling,Liu, Conghui,et al. The polymorphisms in the promoter of HSP90 gene and their association with heat tolerance of bay scallop[J]. CELL STRESS & CHAPERONES,2015,20(2):297-308. |
| APA | Yang, Chuanyan,Wang, Lingling,Liu, Conghui,Zhou, Zhi,Zhao, Xin,&Song, Linsheng.(2015).The polymorphisms in the promoter of HSP90 gene and their association with heat tolerance of bay scallop.CELL STRESS & CHAPERONES,20(2),297-308. |
| MLA | Yang, Chuanyan,et al."The polymorphisms in the promoter of HSP90 gene and their association with heat tolerance of bay scallop".CELL STRESS & CHAPERONES 20.2(2015):297-308. |
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