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Hydrogen production by Enterobacter cloacae isolated from sugar refinery sludge
Sun, Lili1; Huang, Aiyou2; Gu, Wenhui2; Ma, Yingchao2; Zhu, Daling1; Wang, Guangce2
2015-01-21
发表期刊INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷号40期号:3页码:1402-1407
文章类型Article
摘要Hydrogen is recognized as a promising fuel for the future because it is renewable, environmentally harmless and cost-effective. Systematic research on the process of hydrogen production is important for increasing hydrogen yield and identification of suitable strains of bacteria. We investigated the hydrogen production pathways of Enterobacter cloacae. The maximum hydrogen yield was -707 ml L-1, the H-2 content of the gasphase was in the range 30-35% and the overall rate of hydrogen production was -70.70 ml h(-1) L-1. Ethyl alcohol and 2,3-butanediol were the major fermentative products, indicating the hydrogen-producing fermentation pathway of E. cloacae was the 2,3butanediol pathway. Proteins and enzymes involved in the 2,3-butanediol pathway were generally detected by proteomics analysis, providing further evidence for the presence of the 2,3-butanediol pathway in E. cloacae. Both C-13 labeling experiments and proteomics analysis indicated the Embden-Meyerhof-Pamas (EMP) pathway was the major pathway and the hexose monophosphate (HMP) pathway had a weaker role, or did not participate, in glucose catalysis in E. cloacae. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
关键词Hydrogen Production Enterobacter Cloacae 2 Proteomics Analysis 3-butanediol Pathway C-13 Labeling Experiment
DOI10.1016/j.ijhydene.2014.11.121
收录类别SCI
语种英语
WOS记录号WOS:000349060100006
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/25344
专题实验海洋生物学重点实验室
作者单位1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
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GB/T 7714
Sun, Lili,Huang, Aiyou,Gu, Wenhui,et al. Hydrogen production by Enterobacter cloacae isolated from sugar refinery sludge[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2015,40(3):1402-1407.
APA Sun, Lili,Huang, Aiyou,Gu, Wenhui,Ma, Yingchao,Zhu, Daling,&Wang, Guangce.(2015).Hydrogen production by Enterobacter cloacae isolated from sugar refinery sludge.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,40(3),1402-1407.
MLA Sun, Lili,et al."Hydrogen production by Enterobacter cloacae isolated from sugar refinery sludge".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 40.3(2015):1402-1407.
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