Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers

IOCAS-IR > 海洋环流与波动重点实验室

	Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers
	Shi, CJ ; Hu, ZM ; He, YJ ; Zou, YP ; Zhang, DM ; Duan, DL
	2005-03-01
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	47 期号:3 页码:283-291
文章类型	Article
摘要	Molecular markers were used to identify and assess cultivars of Laminaria Lamx. and to delineate their phylogenetic relationships. Random amplified polymorphic DNA (RAPD) analysis was used for detection. After screening, 11 primers were selected and they yielded 133 bands in all, of which approximately 99.2% were polymorphic. The genetic distances between gametophytes ranged from 0.412 to 0.956. Two clusters were formed with the unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the simple matching coefficient. All cultivars of Laminaria japonica Aresch. used for breeding in China fell into one cluster. L. japonica from Japan, L. saccharina (L.) Lam., and L. angustata Kjellm. formed the other cluster and showed higher genetic variation than L. japonica from China. Nuclear ribosomal DNA (rDNA) sequences, including internal transcribed spacers (ITS1 and ITS2) were studied and aligned. The nucleotides of the sequences ranged from 634 to 668, with a total of 692 positions including TTS1, ITS2, and the 5.8S coding region. The phylogenetic tree obtained by the neighbor-joining method favored, to some extent, the results revealed by RAPD analysis. The present study indicates that RAPD and ITS analyses could be used to identify and assess Laminaria germplasm and to distinguish some species and, even intraspecies, in Laminaria.; Molecular markers were used to identify and assess cultivars of Laminaria Lamx. and to delineate their phylogenetic relationships. Random amplified polymorphic DNA (RAPD) analysis was used for detection. After screening, 11 primers were selected and they yielded 133 bands in all, of which approximately 99.2% were polymorphic. The genetic distances between gametophytes ranged from 0.412 to 0.956. Two clusters were formed with the unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the simple matching coefficient. All cultivars of Laminaria japonica Aresch. used for breeding in China fell into one cluster. L. japonica from Japan, L. saccharina (L.) Lam., and L. angustata Kjellm. formed the other cluster and showed higher genetic variation than L. japonica from China. Nuclear ribosomal DNA (rDNA) sequences, including internal transcribed spacers (ITS1 and ITS2) were studied and aligned. The nucleotides of the sequences ranged from 634 to 668, with a total of 692 positions including TTS1, ITS2, and the 5.8S coding region. The phylogenetic tree obtained by the neighbor-joining method favored, to some extent, the results revealed by RAPD analysis. The present study indicates that RAPD and ITS analyses could be used to identify and assess Laminaria germplasm and to distinguish some species and, even intraspecies, in Laminaria.
关键词	Genetic Variation Internal Transcribed Spacers (Its) Laminaria Lamx. Phylogenesis Random Amplified Polymorphic Dna (Rapd)
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000227555900004
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2448
专题	海洋环流与波动重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Shi, CJ,Hu, ZM,He, YJ,et al. Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2005,47(3):283-291.
APA	Shi, CJ,Hu, ZM,He, YJ,Zou, YP,Zhang, DM,&Duan, DL.(2005).Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,47(3),283-291.
MLA	Shi, CJ,et al."Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 47.3(2005):283-291.

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