| The application of multiple miRNA response elements enables oncolytic adenoviruses to possess specificity to glioma cells | |
| Yao, Weicheng1; Guo, Guocai1; Zhang, Quanqin2; Fan, Lingyan3; Wu, Ning4; Bo, Yongli1; Bo, YL (reprint author), Qingdao Univ, Affiliated Hosp, Coll Med, Dept Neurosurg, Qingdao 266003, Peoples R China. | |
| 2014-06-01 | |
| 发表期刊 | VIROLOGY
 |
| 卷号 | 458页码:69-82 |
| 文章类型 | Article |
| 摘要 | Adenovirus-mediated virotherapy is one of the promising therapeutic approaches for glioma treatment. However, its replication efficiency and specificity still failed to meet the requirements for clinical treatment. To improve the anti-tumor activity and specificity of oncolytic adenoviruses (OA), we applied multiple miRNA response elements (MREs) of miR-124, miR-128, miR-146b and miR-218, whose expressions were downregulated in glioma cells, to enable OA to be specific to glioma. Adenoviral E1A protein regulated by these 4 MREs (OA-4MREs) was shown to be highly expressed in glioma cells, but not in normal cells. The selective ElA expression led to glioma-specific replication and cytotoxicity of OA-4MREs. Animal experiments also showed that OA-4MREs exhibited improved anti-tumor activities for both subcutaneous and intracranial glioma xenografts, without significant toxicity to normal brain and liver tissues. Collectively, we demonstrated that oncolytic adenovirus, whose replication was regulated by MREs, may be promising biological agents for glioma treatment. (C) 2014 Elsevier Inc. All rights reserved. |
| 关键词 | Adenovirus Glioma Mirna |
| 学科领域 | Virology |
| DOI | 10.1016/j.virol.2014.04.007 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337664800008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24245 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Bo, YL (reprint author), Qingdao Univ, Affiliated Hosp, Coll Med, Dept Neurosurg, Qingdao 266003, Peoples R China. |
| 作者单位 | 1.Qingdao Univ, Affiliated Hosp, Coll Med, Dept Neurosurg, Qingdao 266003, Peoples R China 2.Qingdao Univ, Affiliated Hosp, Coll Med, Dept Internal Med, Qingdao 266003, Peoples R China 3.Qingdao Univ, Affiliated Hosp, Coll Med, Dept Neurol, Qingdao 266003, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Weicheng,Guo, Guocai,Zhang, Quanqin,et al. The application of multiple miRNA response elements enables oncolytic adenoviruses to possess specificity to glioma cells[J]. VIROLOGY,2014,458:69-82. |
| APA | Yao, Weicheng.,Guo, Guocai.,Zhang, Quanqin.,Fan, Lingyan.,Wu, Ning.,...&Bo, YL .(2014).The application of multiple miRNA response elements enables oncolytic adenoviruses to possess specificity to glioma cells.VIROLOGY,458,69-82. |
| MLA | Yao, Weicheng,et al."The application of multiple miRNA response elements enables oncolytic adenoviruses to possess specificity to glioma cells".VIROLOGY 458(2014):69-82. |
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