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Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain
Feng, Xinjun1,2; Ding, Yamei3; Xian, Mo1; Xu, Xin1; Zhang, Rubing1; Zhao, Guang1; Zhao, G (reprint author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China.
2014-11-01
Source PublicationBIORESOURCE TECHNOLOGY
Volume172Pages:269-275
SubtypeArticle
AbstractIn this study, glycerol was used to produce optically pure D-lactate by engineered Klebsiella pneumoniae strain. In the recombinant strain, D-lactate dehydrogenase LdhA was overexpressed, and two genes, dhaT and yqhD for biosynthesis of main byproduct 1,3-propanediol, were knocked out. To further improve D-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in D-lactate production. In microaerobic fed-batch fermentation, the engineered strain accumulated 142.1 g/L optically pure D-lactate with a yield of 0.82 g/g glycerol, which represented the highest D-lactate production from glycerol so far. This study showed that K. pneumoniae strain has high efficiency to convert glycerol into D-lactate and high potentiality in utilization of crude glycerol from biodiesel industry. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordD-lactate Glycerol Engineered Klebsiella Pneumoniae Strain Bioconversion
Subject AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
DOI10.1016/j.biortech.2014.09.074
Indexed BySCI
Language英语
WOS IDWOS:000344113800037
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/24238
Collection海洋生物技术研发中心
Corresponding AuthorZhao, G (reprint author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China.
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Feng, Xinjun,Ding, Yamei,Xian, Mo,et al. Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain[J]. BIORESOURCE TECHNOLOGY,2014,172:269-275.
APA Feng, Xinjun.,Ding, Yamei.,Xian, Mo.,Xu, Xin.,Zhang, Rubing.,...&Zhao, G .(2014).Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain.BIORESOURCE TECHNOLOGY,172,269-275.
MLA Feng, Xinjun,et al."Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain".BIORESOURCE TECHNOLOGY 172(2014):269-275.
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