| Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain | |
Feng, Xinjun1,2; Ding, Yamei3; Xian, Mo1; Xu, Xin1 ; Zhang, Rubing1; Zhao, Guang1; Zhao, G (reprint author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China.
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| 2014-11-01 | |
| 发表期刊 | BIORESOURCE TECHNOLOGY
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| 卷号 | 172页码:269-275 |
| 文章类型 | Article |
| 摘要 | In this study, glycerol was used to produce optically pure D-lactate by engineered Klebsiella pneumoniae strain. In the recombinant strain, D-lactate dehydrogenase LdhA was overexpressed, and two genes, dhaT and yqhD for biosynthesis of main byproduct 1,3-propanediol, were knocked out. To further improve D-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in D-lactate production. In microaerobic fed-batch fermentation, the engineered strain accumulated 142.1 g/L optically pure D-lactate with a yield of 0.82 g/g glycerol, which represented the highest D-lactate production from glycerol so far. This study showed that K. pneumoniae strain has high efficiency to convert glycerol into D-lactate and high potentiality in utilization of crude glycerol from biodiesel industry. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | D-lactate Glycerol Engineered Klebsiella Pneumoniae Strain Bioconversion |
| 学科领域 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| DOI | 10.1016/j.biortech.2014.09.074 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344113800037 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24238 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Zhao, G (reprint author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Xinjun,Ding, Yamei,Xian, Mo,et al. Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain[J]. BIORESOURCE TECHNOLOGY,2014,172:269-275. |
| APA | Feng, Xinjun.,Ding, Yamei.,Xian, Mo.,Xu, Xin.,Zhang, Rubing.,...&Zhao, G .(2014).Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain.BIORESOURCE TECHNOLOGY,172,269-275. |
| MLA | Feng, Xinjun,et al."Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain".BIORESOURCE TECHNOLOGY 172(2014):269-275. |
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