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Molecular Cloning of Hemocyanin cDNA from Fenneropenaeus chinensis and Antimicrobial Analysis of Two C-terminal Fragments
Qiu, Chuwen1,2; Sun, Jie3; Liu, Mei1; Wang, Baojie1; Jiang, Keyong1; Sun, Shujuan1; Meng, Xiaolin1,2; Luo, Zuoyong1,2; Wang, Lei1; Liu, M (reprint author), Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2014-02-01
发表期刊MARINE BIOTECHNOLOGY
卷号16期号:1页码:46-53
文章类型Article
摘要Peptides derived from shrimp hemocyanin have antimicrobial properties. This is the first report of hemocyanin cDNA (FCHc) cloned from Fenneropenaeus chinensis and recombinant expression of two C-terminal fragments. Based on sequence analysis of Fenneropenaeus chinensis hemocyanin FCHc, we subcloned two FCHc fragments by designing special primers. Two antimicrobial peptides (AMPs) were derived from FCHc (FCHc-C1 and FCHc-C2). The recombinant sequence of FCHc-C1 consisted of 207 bp encoding 69 amino acids and the recombinant sequence of FCHc-C2 consisted of 120 bp encoding 40 amino acids. The results of Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting indicated that recombinant FCHc-C1 and FCHc-C2 peptides (rFCHc-C1 and rFCHc-C2) were expressed successfully. An inhibition assay showed that FCHc-C1 and FCHc-C2 were anionic AMPs with antifungal and antibacterial activities.
关键词Fenneropenaeus Chinensis Hemocyanin Antimicrobial Peptides (Amps) Pichia Pastoris Recombinant Expression
学科领域Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI10.1007/s10126-013-9519-y
收录类别SCI
语种英语
WOS记录号WOS:000330433700005
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被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/24234
专题海洋生物技术研发中心
通讯作者Liu, M (reprint author), Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China
第一作者单位中国科学院海洋研究所
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Qiu, Chuwen,Sun, Jie,Liu, Mei,et al. Molecular Cloning of Hemocyanin cDNA from Fenneropenaeus chinensis and Antimicrobial Analysis of Two C-terminal Fragments[J]. MARINE BIOTECHNOLOGY,2014,16(1):46-53.
APA Qiu, Chuwen.,Sun, Jie.,Liu, Mei.,Wang, Baojie.,Jiang, Keyong.,...&Liu, M .(2014).Molecular Cloning of Hemocyanin cDNA from Fenneropenaeus chinensis and Antimicrobial Analysis of Two C-terminal Fragments.MARINE BIOTECHNOLOGY,16(1),46-53.
MLA Qiu, Chuwen,et al."Molecular Cloning of Hemocyanin cDNA from Fenneropenaeus chinensis and Antimicrobial Analysis of Two C-terminal Fragments".MARINE BIOTECHNOLOGY 16.1(2014):46-53.
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