| Jellyfish venomics and venom gland transcriptomics analysis of Stomolophus meleagris to reveal the toxins associated with sting | |
Li, Rongfeng1; Yu, Huahua1 ; Xue, Wei1,2; Yue, Yang1,2; Liu, Song1; Xing, Ronge1; Li, Pengcheng1; Li, PC (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2014-06-25 | |
| 发表期刊 | JOURNAL OF PROTEOMICS
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| 卷号 | 106页码:17-29 |
| 文章类型 | Article |
| 摘要 | Jellyfish Stomolophus meleagris is a very dangerous animal because of its strong toxicity. However, the composition of the venom is still unclear. Both proteomics and transcriptomics approaches were applied in present study to investigate the major components and their possible relationships to the sting. The proteomics of the venom from S. meleagris was conducted by tryptic digestion of the crude venom followed by RP-HPLC separation and MS/MS analysis of the tryptic peptides. The venom gland transcriptome was analyzed using a high-throughput Illumina sequencing platform HiSeq (TM) 2000 with de novo assembly. A total of 218 toxins were identified including C-type lectin, phospholipase A(2) (PLA(2)), potassium channel inhibitor, protease inhibitor, metalloprotease, hemolysin and other toxins, most of which should be responsible for the sting. Among them, serine protease inhibitor, PLA(2), potassium channel inhibitor and metalloprotease are predominant, representing 28.44%, 21.56%, 16.06% and 15.14% of the identified venom proteins, respectively. Overall, our combined proteomics and transcriptomics approach provides a systematic overview of the toxins in the venom of jellyfish S. meleagris and it will be significant to understand the mechanism of the sting. |
| 关键词 | Jellyfish Stomolophus Meleagris Venomics Transcriptomics Toxin Sting |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1016/j.jprot.2014.04.011 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000338621700002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24230 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 海洋环境工程技术研究发展中心 |
| 通讯作者 | Li, PC (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Rongfeng,Yu, Huahua,Xue, Wei,et al. Jellyfish venomics and venom gland transcriptomics analysis of Stomolophus meleagris to reveal the toxins associated with sting[J]. JOURNAL OF PROTEOMICS,2014,106:17-29. |
| APA | Li, Rongfeng.,Yu, Huahua.,Xue, Wei.,Yue, Yang.,Liu, Song.,...&Li, PC .(2014).Jellyfish venomics and venom gland transcriptomics analysis of Stomolophus meleagris to reveal the toxins associated with sting.JOURNAL OF PROTEOMICS,106,17-29. |
| MLA | Li, Rongfeng,et al."Jellyfish venomics and venom gland transcriptomics analysis of Stomolophus meleagris to reveal the toxins associated with sting".JOURNAL OF PROTEOMICS 106(2014):17-29. |
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