| Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas | |
Song, Xiaorui1,2 ; Wang, Xiaotong1; Li, Li1; Zhang, Guofan1; Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China.
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| 2014-07-01 | |
| 发表期刊 | MOLECULAR BIOLOGY REPORTS
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| 卷号 | 41期号:7页码:4273-4278 |
| 文章类型 | Article |
| 摘要 | Nacrein-like proteins have carbonic anhydrase (CA)-like domains, but their coding regions are flanked by inserted repeat sequence, such as Gly-X-Asn. Reportedly, nacrein-like proteins show the highest similarity to human carbonic anhydrase 1(alpha-CA1), possess CA catalytic functions, and play a key role in shell biomineralization. In the present study, two novel nacrein-like proteins were firstly identified from the shell-forming mantle of the Pacific oyster Crassostrea gigas. With numerous analyses, it was identified and characterized that both the nacrein-like proteins F1 and F2 were secreted and most closely related to the nacrein-like protein of California mussel Mytilus californianus via phylogenetic analysis. RT-PCR analysis showed that the nacrein-like proteins F1 and F2 were expressed in multiple tissues and the expression levels remarkably rose after entering the spat stage, which were basically consistent with the increase of calcite fractions in the total shell volume. Surprisingly, the Gly-X-Asn repeat domain, which is distinctive in most nacrein-like proteins, was absent in the two newly identified nacrein-like proteins in C. gigas and replaced with a series of acidic amino acids (D/E). Regardless, nacrein-like proteins in mollusks seem to be vital to the deposition of calcium carbonate and likely perform diverse functions. |
| 关键词 | Nacrein-like Proteins Shell Biomineralization Crassostrea Gigas Calcite Fractions |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1007/s11033-014-3298-z |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000338283000012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24224 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Song, Xiaorui,Wang, Xiaotong,Li, Li,et al. Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas[J]. MOLECULAR BIOLOGY REPORTS,2014,41(7):4273-4278. |
| APA | Song, Xiaorui,Wang, Xiaotong,Li, Li,Zhang, Guofan,&Zhang, GF .(2014).Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas.MOLECULAR BIOLOGY REPORTS,41(7),4273-4278. |
| MLA | Song, Xiaorui,et al."Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas".MOLECULAR BIOLOGY REPORTS 41.7(2014):4273-4278. |
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