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Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum
Ding, Jianfeng1,2; Wang, Rui1,2; Yang, Feng1,2; Zhao, Liqiang1,2; Qin, Yanjie1,2; Zhang, Guofan3; Yan, Xiwu1,2; Yan, XW (reprint author), Dalian Ocean Univ, Dalian 116023, Peoples R China.
2014-11-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号47期号:1页码:81-89
文章类型Article
摘要A novel lysozyme gene (RpLysPh) with high similarity to the bacteriophage lysozymes was identified in Manila clam, Ruditapes philippinarum. The full length cDNA of RpLysPh is 828 bp and contains a 462 bp open reading frame (ORF) that codes for a 154 amino acid protein. Multiple sequence alignment analysis revealed that the three residues essential for catalytic activity in phage-type lysozyme (Glu(20), Asp(29), and Thr(35)) are conserved in RpLysPh. The comparison of the 3D models of RpLysPh and Coxiella burnetii lysozyme also suggested that the active sites involved in the binding of substrate have similar conformations. Phylogenetic analysis suggested that RpLysPh shares a similar origin with the bacterial phage-type lysozyme group. The highest level of expression of RpLysPh was observed in hemocytes, followed by mantle. Induction of RpLysPh expression was observed in gills in response to lipopolysaccharide (LPS), peptidoglycan (PGN), polyinosinic-polycytidylic acid (Poly(I:C)), and whole glucan particles (WGP) challenge. The recombinant protein of RpLysPh showed antibacterial activity against both Gram-positive and Gram-negative bacteria. (C) 2014 Elsevier Ltd. All rights reserved.
关键词Ruditapes Philippinarum Phage-type Like Lysozyme Basal And Temporal Transcriptional Analysis Antibacterial Activity
学科领域Immunology ; Zoology
DOI10.1016/j.dci.2014.06.013
收录类别SCI
语种英语
WOS记录号WOS:000342275400010
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/24221
专题海洋生物技术研发中心
通讯作者Yan, XW (reprint author), Dalian Ocean Univ, Dalian 116023, Peoples R China.
作者单位1.Dalian Ocean Univ, Dalian 116023, Peoples R China
2.Engn Res Ctr Shellfish Culture & Breeding Liaonin, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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Ding, Jianfeng,Wang, Rui,Yang, Feng,et al. Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,47(1):81-89.
APA Ding, Jianfeng.,Wang, Rui.,Yang, Feng.,Zhao, Liqiang.,Qin, Yanjie.,...&Yan, XW .(2014).Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,47(1),81-89.
MLA Ding, Jianfeng,et al."Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 47.1(2014):81-89.
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