IOCAS-IR  > 海洋生物技术研发中心
Functional characterization of a Delta 5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve
Liu, Helu1,2; Guo, Zhicheng1,2; Zheng, Huaiping1,2; Wang, Shuqi1,2; Wang, Yajun1,2; Liu, Wenhua1,2; Zhang, Guofan3; Zheng, HP (reprint author), Mariculture Res Ctr Subtrop Shellfish & Algae, Dept Educ Guangdong Prov, Shantou 515063, Peoples R China.
2014-11-01
发表期刊MOLECULAR BIOLOGY REPORTS
卷号41期号:11页码:7437-7445
文章类型Article
摘要Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in lots of important physiological processes, while, many marine species have no or limited ability of endogenous PUFA biosynthesis, normally due to the lack of key enzymes such as fatty acid desaturase (FAD). In this study, we isolated a scallop Chlamys nobilis cDNA with high homology to vertebrate FADs. Functional characterization in recombinant yeast Saccharomyces cerevisiae showed that scallop FAD exhibited Delta 5-desaturation activity towards both saturated and PUFA substrates. Thus, it efficiently desaturated exogenously added PUFA C20:4(n - 3) and C20:3(n - 6) to C20:5(n - 3) (EPA) and C20:4(n - 6) (ARA) respectively. It also converted the yeast's endogenous C18:0 into C18:1(n - 13), and participated in the biosynthesis of non-methylene-interrupted FA by introducing a double bond to C20:3(n - 3) and C20:2(n - 6) in the Delta 5 carbon. Temporal transcript profile of scallop FAD was studied during early embryonic development. High level of mRNA was found at the beginning of embryogenesis (egg) and noticeable decreases of were observed during larvae development, suggesting maternal FAD mRNA transfer to the embryo. Further, FAD transcripts were detected in all tissues analyzed, with the gonad and hepatopancreas showing the highest expression.
关键词Delta 5 Fatty Acid Desaturase Chlamys Nobilis Pufa Biosynthesis Larvae Development Marine Bivalves
学科领域Biochemistry & Molecular Biology
DOI10.1007/s11033-014-3633-4
收录类别SCI
语种英语
WOS记录号WOS:000344101300044
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/24214
专题海洋生物技术研发中心
通讯作者Zheng, HP (reprint author), Mariculture Res Ctr Subtrop Shellfish & Algae, Dept Educ Guangdong Prov, Shantou 515063, Peoples R China.
作者单位1.Shantou Univ, Key Lab Marine Biotechnol Guangdong Prov, Shantou 515063, Peoples R China
2.Mariculture Res Ctr Subtrop Shellfish & Algae, Dept Educ Guangdong Prov, Shantou 515063, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Liu, Helu,Guo, Zhicheng,Zheng, Huaiping,et al. Functional characterization of a Delta 5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve[J]. MOLECULAR BIOLOGY REPORTS,2014,41(11):7437-7445.
APA Liu, Helu.,Guo, Zhicheng.,Zheng, Huaiping.,Wang, Shuqi.,Wang, Yajun.,...&Zheng, HP .(2014).Functional characterization of a Delta 5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve.MOLECULAR BIOLOGY REPORTS,41(11),7437-7445.
MLA Liu, Helu,et al."Functional characterization of a Delta 5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve".MOLECULAR BIOLOGY REPORTS 41.11(2014):7437-7445.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Functional character(1469KB) 限制开放ODC PDDL浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Helu]的文章
[Guo, Zhicheng]的文章
[Zheng, Huaiping]的文章
百度学术
百度学术中相似的文章
[Liu, Helu]的文章
[Guo, Zhicheng]的文章
[Zheng, Huaiping]的文章
必应学术
必应学术中相似的文章
[Liu, Helu]的文章
[Guo, Zhicheng]的文章
[Zheng, Huaiping]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Functional characterization of a Delta 5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。