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Alternative splicing and immune response of Crassostrea gigas tumor necrosis factor receptor-associated factor 3
Huang, Baoyu1,2; Zhang, Linlin1; Du, Yishuai1; Li, Li1; Qu, Tao1; Meng, Jie1; Zhang, Guofan1; Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2014-10-01
发表期刊MOLECULAR BIOLOGY REPORTS
卷号41期号:10页码:6481-6491
文章类型Article
摘要Diverse alternative splicing isoforms play an important role in immune diversity and specificity. Their role in molluscan host-defense is however poorly understood. We characterized two alternative isoforms of tumor necrosis factor receptor-associated factor 3 (TRAF3) in the Pacific oyster, Crassostrea gigas, which were named CgTRAF3-S and CgTRAF3-L. An intron was retained in CgTRAF3-L, introducing a premature termination codon. Comparison and phylogenetic analysis revealed that CgTRAF3 shared a higher identity with other species, suggesting the conservation of the two gene transcripts. Quantitative real-time PCR was performed and the expression levels of CgTRAF3 isoforms were found to be significantly changed after Vibrio anguillarum and ostreid herpesvirus 1 challenges. These two isoforms represented contrary trends, indicating that CgTRAF3-L might function as a negative regulator of CgTRAF3-S. We also investigated the expression level of the transcripts of the two CgTRAF3 isoforms, following the silence of C. gigas mitochondrial anti-viral signaling protein like gene (CgMAVS-like). We concluded that CgTRAF3 might be involved in a MAVS-mediated immune signaling pathway. This study suggests that CgTRAF3 may be a response to bacterial and viral stimulation and that the two isoforms may be involved in immune response pathways. It is also possible that the two alternative splicing isoforms could be inter-coordinated and may promote survival of these oysters under immune stress conditions.
关键词Crassostrea Gigas Traf3 Alternative Splicing Immune Response
学科领域Biochemistry & Molecular Biology
DOI10.1007/s11033-014-3531-9
收录类别SCI
语种英语
WOS记录号WOS:000342440000019
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/24195
专题海洋生物技术研发中心
实验海洋生物学重点实验室
通讯作者Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Huang, Baoyu,Zhang, Linlin,Du, Yishuai,et al. Alternative splicing and immune response of Crassostrea gigas tumor necrosis factor receptor-associated factor 3[J]. MOLECULAR BIOLOGY REPORTS,2014,41(10):6481-6491.
APA Huang, Baoyu.,Zhang, Linlin.,Du, Yishuai.,Li, Li.,Qu, Tao.,...&Li, L .(2014).Alternative splicing and immune response of Crassostrea gigas tumor necrosis factor receptor-associated factor 3.MOLECULAR BIOLOGY REPORTS,41(10),6481-6491.
MLA Huang, Baoyu,et al."Alternative splicing and immune response of Crassostrea gigas tumor necrosis factor receptor-associated factor 3".MOLECULAR BIOLOGY REPORTS 41.10(2014):6481-6491.
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