| A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis | |
| Wang Jiafeng1,2; Xu Fei1; Li Li1; Zhang Guofan1; Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China. | |
| 2014-03-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 卷号 | 32期号:2页码:419-425 |
| 文章类型 | Article |
| 摘要 | The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetic studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I (COI) of five common Crassostrea species in China (including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas) and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers (Oyster-COI-1), we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species. |
| 关键词 | Identification Cytochrome Oxidase Subunit i (Coi) Crassostrea Oyster High-resolution Melting (Hrm) |
| 学科领域 | Marine & Freshwater Biology ; Oceanography |
| DOI | 10.1007/s00343-014-3124-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332739600019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24194 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang Jiafeng,Xu Fei,Li Li,et al. A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):419-425. |
| APA | Wang Jiafeng,Xu Fei,Li Li,Zhang Guofan,&Zhang, GF .(2014).A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),419-425. |
| MLA | Wang Jiafeng,et al."A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):419-425. |
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