Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of grass carp against grass carp reovirus | |
| Zhu, Bin1; Liu, Guang-Lu2; Gong, Yu-Xin3; Ling, Fei1; Song, Lin-Sheng4; Wang, Gao-Xue1; Wang, GX (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China. | |
| 2014-12-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| 卷号 | 41期号:2页码:279-293 |
| 文章类型 | Article |
| 摘要 | Grass carp reovirus (GCRV), the most pathogenic aquareovirus, can cause fatal hemorrhagic disease in fingerling and yearling grass carp. Vaccination by injection is by far the most effective method of combating disease. However it is labor intensive, costly and not feasible to vaccinate large numbers of the fish. Thus, an efficient and economic strategy for the prevention of GCRV infection becomes urgent. Here, functionalized single-walled carbon nanotubes (SWCNTs) as carrier were used to manufacture SWCNTs-VP7 subunit vaccine with chemical modification. Different developmental stages of grass carps were immunized by VP7/SWCNTs-VP7 subunit vaccine against GCRV by intramuscular injection and bath immunization. The results indicate that better immune responses of grass carp immunized with the SWCNTs-VP7 subunit vaccine were induced in comparison with VP7 subunit vaccine alone. Immunization doses/concentrations are significantly reduced (about 5-8 times) to prevent GCRV infection in different developmental stages of grass carp with injection or bath treatment when SWCNTs carrier was used. A good immune protective effect (relative percentage survival greater than 95%) is observed in smaller size fish (0.2 g) with SWCNT5-VP7 bath immunization. In addition, serum respiratory burst activity, complement activity, lysozyme activity, superoxide dismutase activity, alkaline phosphatase activity, immune-related genes and antibody levels were significantly enhanced in fish immunized with vaccine. This study suggested that functionalized SWCNTs was the promising carrier for recombinant subunit vaccine and might be used to vaccinate fish by bath approach. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | Single-walled Carbon Nanotubes Grass Carp Grass Carp Reovirus Immune Response Vaccine |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2014.09.014 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347017500020 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24167 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, GX (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China. |
| 作者单位 | 1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 2.Northwest A&F Univ, Coll Sci, Yangling 712100, Peoples R China 3.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Bin,Liu, Guang-Lu,Gong, Yu-Xin,et al. Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of grass carp against grass carp reovirus[J]. FISH & SHELLFISH IMMUNOLOGY,2014,41(2):279-293. |
| APA | Zhu, Bin.,Liu, Guang-Lu.,Gong, Yu-Xin.,Ling, Fei.,Song, Lin-Sheng.,...&Wang, GX .(2014).Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of grass carp against grass carp reovirus.FISH & SHELLFISH IMMUNOLOGY,41(2),279-293. |
| MLA | Zhu, Bin,et al."Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of grass carp against grass carp reovirus".FISH & SHELLFISH IMMUNOLOGY 41.2(2014):279-293. |
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