Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Ror2 Receptor Mediates Wnt11 Ligand Signaling and Affects Convergence and Extension Movements in Zebrafish | |
Bai, Yan1,2; Tan, Xungang3 ; Zhang, Haifeng1,2; Liu, Chengdong1,2; Zhao, Beibei1,2; Li, Yun1,2; Lu, Ling1,2; Liu, Yunzhang1,2; Zhou, Jianfeng1,2; Zhou, JF (reprint author), Ocean Univ China, Chinese Minist Educ, Key Lab Marine Drugs, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China.
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| 2014-07-25 | |
| 发表期刊 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 卷号 | 289期号:30页码:20664-20676 |
| 文章类型 | Article |
| 摘要 | The receptor-tyrosine kinase Ror2 acts as an alternative receptor or co-receptor for Wnt5a and mediates Wnt5a-induced convergent extension movements during embryogenesis in mice and Xenopus as well as the polarity and migration of several cell types during development. However, little is known about whether Ror2 function is conserved in other vertebrates or is involved in other non-canonical Wnt ligands in vivo. In this study we demonstrated that overexpression of dominant-negative ror2 (ror2-TM) mRNA in zebrafish embryos resulted in convergence and extension defects and incompletely separated eyes, which is consistent with observations from slb/wnt11 mutants or wnt11 knockdown morphants. Moreover, the co-injection of ror2-TM mRNA and a wnt11 morpholino or the coexpression of ror2 and wnt11 in zebrafish embryos synergetically induced more severe convergence and extension defects. Transplantation studies further demonstrated that the Ror2 receptor responded to the Wnt11 ligand and regulated cell migration and cell morphology during gastrulation. DnRor2 inhibited the action of Wnt11, which was revealed by a decreased percentage of Wnt11-induced convergence and extension defects. Ror2 physically interacts with Wnt11. The intracellular Tyr-647 and Ser-863 sites of Ror2 are essential for mediating the action of Wnt11. Dishevelled and RhoA act downstream of Wnt11-Ror2 to regulate convergence and extension movements. Overall, our data suggest an important role of Ror2 in mediating Wnt11 signaling and in regulating convergence and extension movements in zebrafish. |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1074/jbc.M114.586099 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339396600025 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24161 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Zhou, JF (reprint author), Ocean Univ China, Chinese Minist Educ, Key Lab Marine Drugs, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China. |
| 作者单位 | 1.Ocean Univ China, Chinese Minist Educ, Key Lab Marine Drugs, Qingdao 266003, Shandong, Peoples R China 2.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Yan,Tan, Xungang,Zhang, Haifeng,et al. Ror2 Receptor Mediates Wnt11 Ligand Signaling and Affects Convergence and Extension Movements in Zebrafish[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2014,289(30):20664-20676. |
| APA | Bai, Yan.,Tan, Xungang.,Zhang, Haifeng.,Liu, Chengdong.,Zhao, Beibei.,...&Zhou, JF .(2014).Ror2 Receptor Mediates Wnt11 Ligand Signaling and Affects Convergence and Extension Movements in Zebrafish.JOURNAL OF BIOLOGICAL CHEMISTRY,289(30),20664-20676. |
| MLA | Bai, Yan,et al."Ror2 Receptor Mediates Wnt11 Ligand Signaling and Affects Convergence and Extension Movements in Zebrafish".JOURNAL OF BIOLOGICAL CHEMISTRY 289.30(2014):20664-20676. |
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