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Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus)
Wang, Xianlei1,2; Tan, Xungang1; Zhang, Pei-Jun1; Zhang, Yuqing1; Xu, Peng1; Tan, XG (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2014-12-01
Source PublicationJOURNAL OF BIOSCIENCES
Volume39Issue:5Pages:849-858
SubtypeArticle
AbstractDuring the development of B and T lymphocytes, Ig and TCR variable region genes are assembled from germline V, D, and J gene segments by a site-specific recombination reaction known as V(D)J recombination. The process of somatic V(D)J recombination, mediated by the recombination-activating gene (RAG) products, is the most significant characteristic of adaptive immunity in jawed vertebrates. Flounder (Paralichthys olivaceus) RAG1 and RAG2 were isolated by Genome Walker and RT-PCR, and their expression patterns were analysed by RT-PCR and in situ hybridization on sections. RAG1 spans over 7.0 kb, containing 4 exons and 3 introns, and the full-length ORF is 3207 bp, encoding a peptide of 1068 amino acids. The first exon lies in the 5'-UTR, which is an alternative exon. RAG2 full-length ORF is 1062 bp, encodes a peptide of 533 amino acids, and lacks introns in the coding region. In 6-month-old flounders, the expression of RAG1 and RAG2 was essentially restricted to the pronephros (head kidney) and mesonephros (truck kidney). Additionally, both of them were mainly expressed in the thymus. These results revealed that the thymus and kidney most likely serve as the primary lymphoid tissues in the flounder.
KeywordFlounder Rag1 Rag2 Thymus
Subject AreaLife Sciences & Biomedicine - Other Topics
DOI10.1007/s12038-014-9469-1
Indexed BySCI
Language英语
WOS IDWOS:000345809200012
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/24157
Collection实验海洋生物学重点实验室
海洋环流与波动重点实验室
Corresponding AuthorTan, XG (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xianlei,Tan, Xungang,Zhang, Pei-Jun,et al. Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus)[J]. JOURNAL OF BIOSCIENCES,2014,39(5):849-858.
APA Wang, Xianlei,Tan, Xungang,Zhang, Pei-Jun,Zhang, Yuqing,Xu, Peng,&Tan, XG .(2014).Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus).JOURNAL OF BIOSCIENCES,39(5),849-858.
MLA Wang, Xianlei,et al."Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus)".JOURNAL OF BIOSCIENCES 39.5(2014):849-858.
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