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| Photo-biological hydrogen production by an acid tolerant mutant of Rhodovulum sulfidophilum P5 generated by transposon mutagenesis | |
| Cai, Jinling1; Wang, Guangce1,2; Cai, JL (reprint author), Tianjin Univ Sci & Technol, Tianjin Key Lab Marine Resources & Chem, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China. | |
| 2014-02-01 | |
| 发表期刊 | BIORESOURCE TECHNOLOGY
 |
| 卷号 | 154页码:254-259 |
| 文章类型 | Article |
| 摘要 | Most of the photosynthetic bacterial strains exhibit optimum hydrogen production at neutral initial pH, and lower initial pH resulted in a sharp decrease in hydrogen yield. Thus, screening of acid-tolerant hydrogen-producing photosynthetic bacteria is very important. To obtain acid tolerant mutants, a Tn7-based transposon was randomly inserted into the genomic DNA of Rhodovulum sulfidophilum P5. An acid tolerant mutant strain TH-102 exhibited increased hydrogen production in acidic environment (pH 4.5-6.5) and at higher temperatures (35 and 37 degrees C) than the wild-type strain. At pH 5.5 and 35 degrees C, the mutant strain TH-102 continuously produced hydrogen. The hydrogen yield and average rate were 2.16 +/- 0.10 mol/mol acetate and 10.06 +/- 0.47 mL/L h, which was about 17.32 and 15.37-fold higher than that of the wild-type strain, respectively. This acid-and temperature-tolerant mutant strain TH-102 could be used in a cost-effective hydrogen production process employing both dark fermentative and photosynthetic bacteria. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | Hydrogen Production Photosynthetic Bacteria Acid Tolerant Transposon Mutation |
| 学科领域 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| DOI | 10.1016/j.biortech.2013.12.057 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331461600035 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24143 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Cai, JL (reprint author), Tianjin Univ Sci & Technol, Tianjin Key Lab Marine Resources & Chem, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China. |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Tianjin Key Lab Marine Resources & Chem, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Jinling,Wang, Guangce,Cai, JL . Photo-biological hydrogen production by an acid tolerant mutant of Rhodovulum sulfidophilum P5 generated by transposon mutagenesis[J]. BIORESOURCE TECHNOLOGY,2014,154:254-259. |
| APA | Cai, Jinling,Wang, Guangce,&Cai, JL .(2014).Photo-biological hydrogen production by an acid tolerant mutant of Rhodovulum sulfidophilum P5 generated by transposon mutagenesis.BIORESOURCE TECHNOLOGY,154,254-259. |
| MLA | Cai, Jinling,et al."Photo-biological hydrogen production by an acid tolerant mutant of Rhodovulum sulfidophilum P5 generated by transposon mutagenesis".BIORESOURCE TECHNOLOGY 154(2014):254-259. |
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