Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Multiple ferritin subunit genes of the Pacific oyster Crassostrea gigas and their distinct expression patterns during early development | |
Huan, Pin1; Liu, Gang1,2 ; Wang, Hongxia1; Liu, Baozhong1; Liu, BZ (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2014-08-01 | |
| 发表期刊 | GENE
 |
| 卷号 | 546期号:1页码:80-88 |
| 文章类型 | Article |
| 摘要 | Multiple ferritin subunit genes are reported in mollusks, but they have not been systematically classified. Based on the recently published whole genome sequence, we screened out the four ferritin subunit genes (cgi-fer1-cgi-fer4) from the Pacific oyster Crassostrea gigas. The four genes were predicted to encode two non-secretory and two secretory peptides. Further phylogenetic analyses revealed two groups of non-secretory and secretory ferritin subunits in mollusks. This differs dramatically from the situation in mammals or insects, which contain only non-secretory or secretory ferritin subunits. These results emphasize the evolution of molluscan ferritin subunit genes. The expression patterns of the four genes during early development exhibited dramatic differences, indicating the functional diversity of these genes. Among them, cgi-fer2 was the only gene expressed prevalently and is thus suggested to be the major house-keeping ferritin subunit gene. The expression of the other three genes was tissue-specific beginning in the D-veliger stage. Based on their expression patterns, we inferred important functions of cgi-fer2 in ciliated tissues and of the other three genes in the digestive system. Moreover, our results indicated potentially different roles of ferritin subunit genes during larval shell formation in gastropods and bivalves, which may be helpful in understanding the molecular mechanisms that cause the different shells of gastropods and bivalves. In addition, we conducted a further semi-quantitative analysis of the four genes in four major developmental stages and five adult tissues. The results also revealed dramatically different expression patterns of the genes, which brought additional functional indications. This work may promote studies on molluscan ferritins and shed light on the evolution of ferritin subunit genes among different animal groups. (C) 2014 Elsevier B.V. All rights reserved. |
| 关键词 | Bivalve Secretory Ferritin Shell Formation Whole Mount In Situ Hybridization |
| 学科领域 | Genetics & Heredity |
| DOI | 10.1016/j.gene.2014.05.027 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000338412000013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24131 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, BZ (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Huan, Pin,Liu, Gang,Wang, Hongxia,et al. Multiple ferritin subunit genes of the Pacific oyster Crassostrea gigas and their distinct expression patterns during early development[J]. GENE,2014,546(1):80-88. |
| APA | Huan, Pin,Liu, Gang,Wang, Hongxia,Liu, Baozhong,&Liu, BZ .(2014).Multiple ferritin subunit genes of the Pacific oyster Crassostrea gigas and their distinct expression patterns during early development.GENE,546(1),80-88. |
| MLA | Huan, Pin,et al."Multiple ferritin subunit genes of the Pacific oyster Crassostrea gigas and their distinct expression patterns during early development".GENE 546.1(2014):80-88. |
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