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Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in Fenneropenaeus chinensis and its antioxidant activity
Zhang, Qingli1; Huang, Jie1; Li, Fuhua2; Liu, Shuang1; Liu, Qinghui1; Wei, Jiankai2; Liang, Gaofeng1; Xiang, Jianhai2; Xiang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2014-03-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号37期号:1页码:38-45
文章类型Article
摘要Peroxiredoxins (Prx) are a family of antioxidant proteins and perform important functions in intracellular signal transduction. Here, we report a Prx gene from Chinese shrimp Fenneropenaeus chinensis. The full-length cDNA of FcPrx gene contained an open reading frame of 735 bp encoding a polypeptide of 275 amino acids. The molecular mass of the deduced amino acid of FcPrx is 27445.43 Da with an estimated pI of 5.71. Sequence comparison showed that the FcPrx shares high identities with Prx IVs and it was named FcPrx4. A real-Time PCR (qRT-PCR) assay was developed to assess the mRNA expression of FcPrx4 in different tissues and temporal expression in hemocytes and hepatopancreas of E chinensis challenged by white spot syndrome virus (WSSV). Transcripts of FcPrx4 can be detected in all tissues examined. The expression of FcPrx4 showed significant up-regulation in shrimp hemocytes and hepatopancreas after artificial infection with WSSV. A fusion protein containing FcPrx4 was produced in vitro and was confirmed by Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) assay. And activity analysis indicated that the recombinant FcPrx4 proteins can reduce H2O2 in the presence of dithiothreitol. (C) 2014 Elsevier Ltd. All rights reserved.
关键词Peroxiredoxin (Prx) White Spot Syndrome Virus (Wssv) Infection Recombinant Protein Antioxidant Activity
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2013.12.026
收录类别SCI
语种英语
WOS记录号WOS:000333717100005
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/24129
专题实验海洋生物学重点实验室
通讯作者Xiang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
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Zhang, Qingli,Huang, Jie,Li, Fuhua,et al. Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in Fenneropenaeus chinensis and its antioxidant activity[J]. FISH & SHELLFISH IMMUNOLOGY,2014,37(1):38-45.
APA Zhang, Qingli.,Huang, Jie.,Li, Fuhua.,Liu, Shuang.,Liu, Qinghui.,...&Xiang, JH .(2014).Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in Fenneropenaeus chinensis and its antioxidant activity.FISH & SHELLFISH IMMUNOLOGY,37(1),38-45.
MLA Zhang, Qingli,et al."Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in Fenneropenaeus chinensis and its antioxidant activity".FISH & SHELLFISH IMMUNOLOGY 37.1(2014):38-45.
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