Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Mannitol metabolism in brown algae involves a new phosphatase family | |
| Groisillier, Agns1,2; Shao, Zhanru1,2,3; Michel, Gurvan1,2; Goulitquer, Sophie4,5; Bonin, Patricia1,2; Krahulec, Stefan6; Nidetzky, Bernd6; Duan, Delin3; Boyen, Catherine1,2; Tonon, Thierry1,2; Tonon, T (reprint author), Univ Paris 06, UMR Marine Plants & Biomol 7139, Biol Stn, F-29680 Roscoff, France. | |
| 2014-02-01 | |
| 发表期刊 | JOURNAL OF EXPERIMENTAL BOTANY
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| 卷号 | 65期号:2页码:559-570 |
| 文章类型 | Article |
| 摘要 | Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of these is the mannitol cycle, which plays a central role in their physiology, as mannitol acts as carbon storage, osmoprotectant, and antioxidant. This polyol is derived directly from the photoassimilate fructose-6-phosphate via the action of a mannitol-1-phosphate dehydrogenase and a mannitol-1-phosphatase (M1Pase). Genome analysis of the brown algal model Ectocarpus siliculosus allowed identification of genes potentially involved in the mannitol cycle. Among these, two genes coding for haloacid dehalogenase (HAD)-like enzymes were suggested to correspond to M1Pase activity, and thus were named EsM1Pase1 and EsM1Pase2, respectively. To test this hypothesis, both genes were expressed in Escherichia coli. Recombinant EsM1Pase2 was shown to hydrolyse the phosphate group from mannitol-1-phosphate to produce mannitol but was not active on the hexose monophosphates tested. Gene expression analysis showed that transcription of both E. siliculosus genes was under the influence of the diurnal cycle. Sequence analysis and three-dimensional homology modelling indicated that EsM1Pases, and their orthologues in Prasinophytes, should be seen as founding members of a new family of phosphatase with original substrate specificity within the HAD superfamily of proteins. This is the first report describing the characterization of a gene encoding M1Pase activity in photosynthetic organisms. |
| 关键词 | Carbon Storage Ectocarpus Siliculosus Had (Haloacid Dehalogenase) Superfamily Mannitol Phosphatases Primary Metabolism |
| 学科领域 | Plant Sciences |
| DOI | 10.1093/jxb/ert405 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331119300016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24127 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Tonon, T (reprint author), Univ Paris 06, UMR Marine Plants & Biomol 7139, Biol Stn, F-29680 Roscoff, France. |
| 作者单位 | 1.Univ Paris 06, UMR Marine Plants & Biomol 7139, Biol Stn, F-29680 Roscoff, France 2.CNRS, Biol Stn, UMR Marine Plants & Biomol 7139, F-29680 Roscoff, France 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.CNRS, Plate Forme MetaboMER, F-29680 Roscoff, France 5.UPMC, FR2424, Biol Stn, F-29680 Roscoff, France 6.Graz Univ Technol, Inst Biotechnol & Biochem Engn, A-8010 Graz, Austria |
| 推荐引用方式 GB/T 7714 | Groisillier, Agns,Shao, Zhanru,Michel, Gurvan,et al. Mannitol metabolism in brown algae involves a new phosphatase family[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(2):559-570. |
| APA | Groisillier, Agns.,Shao, Zhanru.,Michel, Gurvan.,Goulitquer, Sophie.,Bonin, Patricia.,...&Tonon, T .(2014).Mannitol metabolism in brown algae involves a new phosphatase family.JOURNAL OF EXPERIMENTAL BOTANY,65(2),559-570. |
| MLA | Groisillier, Agns,et al."Mannitol metabolism in brown algae involves a new phosphatase family".JOURNAL OF EXPERIMENTAL BOTANY 65.2(2014):559-570. |
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