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Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens
Zhang, Shu-ren1,2; Zhang, Lu1,2; Sun, Li1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2014-08-11
发表期刊DISEASES OF AQUATIC ORGANISMS
卷号110期号:3页码:181-191
文章类型Article
摘要Pseudomonas fluorescens is a Gram-negative bacterium that can infect a wide range of farmed fish. However, very little is known about the virulence mechanism of P. fluorescens as a fish pathogen. In this study, we identified and analyzed 3 TonB-dependent outer membrane receptors (TDRs) from a pathogenic P. fluorescens strain isolated from fish. In silico analysis revealed that all 3 proteins (named Tdr1 to 3) possess structural domains typical of TDRs. Quantitative real time RT-PCR analysis showed that tdr1, tdr2, and tdr3 expressions were upregulated under iron-depleted conditions. Compared to the wild type, mutants defective in tdr1, tdr2, and tdr3 were retarded in growth to different extents. Infection in a turbot Scophthalmus maximus model showed that all 3 mutants were impaired in their ability to desseminate into and colonize host tissues. In addition, the tdr1 and tdr3 mutants exhibited significantly reduced virulence. When used as subunit vaccines, purified recombinant proteins of Tdr1, Tdr2, and, in particular, Tdr3 elicited significant protection in turbot against lethal P. fluorescens challenge. The vaccinated fish produced specific serum antibodies, which, when incubated with P. fluorescens, blocked infection of P. fluorescens in fish cells. Together these results indicate that Tdr1, Tdr2, and Tdr3 are iron-regulated factors that participate in bacterial virulence and induce protective immunity as subunit vaccines.
关键词Pseudomonas Fluorescens Tonb-dependent Receptor Iron Virulence Vaccine
学科领域Fisheries ; Veterinary Sciences
DOI10.3354/dao02771
收录类别SCI
语种英语
WOS记录号WOS:000342923400003
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/24122
专题实验海洋生物学重点实验室
通讯作者Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位实验海洋生物学重点实验室
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Zhang, Shu-ren,Zhang, Lu,Sun, Li,et al. Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens[J]. DISEASES OF AQUATIC ORGANISMS,2014,110(3):181-191.
APA Zhang, Shu-ren,Zhang, Lu,Sun, Li,&Sun, L .(2014).Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens.DISEASES OF AQUATIC ORGANISMS,110(3),181-191.
MLA Zhang, Shu-ren,et al."Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens".DISEASES OF AQUATIC ORGANISMS 110.3(2014):181-191.
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