Function of shrimp STAT during WSSV infection

doi:10.1016/j.fsi.2014.04.002

IOCAS-IR > 实验海洋生物学重点实验室

	Function of shrimp STAT during WSSV infection
	Wen, Rong 1,2; Li, Fuhua 1; Li, Shihao 1; Xiang, Jianhai1 ; Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2014-06-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	38 期号:2 页码:354-360
文章类型	Article
摘要	JAK/STAT signaling pathway plays key roles in the antiviral immunity of mammals, fish and insect. However, limited knowledge is known about the function of JAK/STAT signaling pathway in the antiviral immunity of shrimp although virus disease has caused severe mortality in shrimp aquaculture. In order to understand the function of JAK/STAT signaling pathway in the antiviral immunity of shrimp, dsRNA interfering technique was used to silence the expression of STAT gene in Litopenaeus vannamei, and the mortality of shrimp was detected after WSSV infection. Furthermore, the expressions of some potential target genes regulated by STAT or genes related to RNA interfering pathway were detected in STAT silenced shrimp during WSSV infection. The WSSV copy number in STAT silenced shrimp was 10(2)-10(3) copies/ng DNA which was much lower than that in the control. The mortality in STAT silenced shrimp caused by WSSV infection decreased very significantly compared to their controls. The function of STAT was verified in vitro cultured cells of hematopoietic tissue of crayfish Cherax quadricarinatus by adding specific inhibitor of STAT3(S3I-201), and the cultured cells treated with S3I-201 showed much less WSSV copy number than their controls, which further suggested that STAT might be helpful for the replication of WSSV. Expression analysis on the potential STAT target genes and genes in RNA interfering pathway provide important information for understanding the functional mechanism of STAT in antiviral immunity of shrimp. (C) 2014 Elsevier Ltd. All rights reserved.
关键词	Stat Rnai Wssv Infection Antiviral Immunity Shrimp
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2014.04.002
收录类别	SCI
语种	英语
WOS记录号	WOS:000337014700011
引用统计	被引频次：66[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24115
专题	实验海洋生物学重点实验室
通讯作者	Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wen, Rong,Li, Fuhua,Li, Shihao,et al. Function of shrimp STAT during WSSV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(2):354-360.
APA	Wen, Rong,Li, Fuhua,Li, Shihao,Xiang, Jianhai,&Li, FH .(2014).Function of shrimp STAT during WSSV infection.FISH & SHELLFISH IMMUNOLOGY,38(2),354-360.
MLA	Wen, Rong,et al."Function of shrimp STAT during WSSV infection".FISH & SHELLFISH IMMUNOLOGY 38.2(2014):354-360.

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