Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions

doi:10.1007/s00343-014-3245-9

IOCAS-IR > 实验海洋生物学重点实验室

	Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions
	Wang Wenlei 1; Wu Xiaojie 1; Wang Chao 2; Jia Zhaojun 1; He Linwen 2; Wei Yifan 2; Niu Jianfeng 2,3; Wang Guangce 1,2; Niu, JF (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
	2014-07-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号	32 期号:4 页码:783-791
文章类型	Article
摘要	To screen the stable expression genes related to the stress (strong light, dehydration and temperature shock) we applied Absolute real-time PCR technology to determine the transcription numbers of the selected test genes in Porphyra yezoensis, which has been regarded as a potential model species responding the stress conditions in the intertidal. Absolute real-time PCR technology was applied to determine the transcription numbers of the selected test genes in Porphyra yezoensis, which has been regarded as a potential model species in stress responding. According to the results of photosynthesis parameters, we observed that Y(II) and F-v/F-m were significantly affected when stress was imposed on the thalli of Porphyra yezoensis, but underwent almost completely recovered under normal conditions, which were collected for the following experiments. Then three samples, which were treated with different grade stresses combined with salinity, irradiation and temperature, were collected. The transcription numbers of seven constitutive expression genes in above samples were determined after RNA extraction and cDNA synthesis. Finally, a general insight into the selection of internal control genes during stress response was obtained. We found that there were no obvious effects in terms of salinity stress (at salinity 90) on transcription of most genes used in the study. The 18S ribosomal RNA gene had the highest expression level, varying remarkably among different tested groups. RPS8 expression showed a high irregular variance between samples. GAPDH presented comparatively stable expression and could thus be selected as the internal control. EF-1 alpha showed stable expression during the series of multiple-stress tests. Our research provided available references for the selection of internal control genes for transcripts determination of P. yezoensis.
关键词	Constitutive Expression Gene Housekeeping Gene Porphyra Yezoensis Ueda Real-time Quantitative Pcr Stress Responding
学科领域	Marine & Freshwater Biology ; Oceanography
DOI	10.1007/s00343-014-3245-9
收录类别	SCI
语种	英语
WOS记录号	WOS:000339046200006
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24112
专题	实验海洋生物学重点实验室
通讯作者	Niu, JF (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
作者单位	1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Nantong Branch, Inst Oceanol, Nantong 226006, Peoples R China
推荐引用方式 GB/T 7714	Wang Wenlei,Wu Xiaojie,Wang Chao,et al. Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(4):783-791.
APA	Wang Wenlei.,Wu Xiaojie.,Wang Chao.,Jia Zhaojun.,He Linwen.,...&Niu, JF .(2014).Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(4),783-791.
MLA	Wang Wenlei,et al."Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.4(2014):783-791.

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