Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus

doi:10.1007/s00343-014-3099-1

IOCAS-IR > 实验海洋生物学重点实验室

	Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus
	Xing Mengxin 1,2; Hou Zhanhui 1; Qu Yanmei 1; Liu Bin 1; Liu, B (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
	2014-03-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号	32 期号:2 页码:316-325
文章类型	Article
摘要	Eighteen agar media were tested for the culture of gut-associated bacteria from farmed adult turbot (Scophthalmus maximus), including 16 agar media with or without 1% gastrointestinal (GI) supernatant, or with 2% or 4% GI supernatant. A total of 1 711 colonies were analyzed and 24 operational taxonomic units (OTUs) were identified. The greatest bacterial diversity was isolated on Zobell 2216E/Zobell 2216E+ agar media, whereas MRS/MRS+ agar media produced a low diversity of colonies. Agar media with GI supernatant (1%, 2%, or 4%) showed increased diversity and yielded different profiles of OTUs from the corresponding original media, suggesting that GI supernatant provides substances that enhance the culture efficiency of bacteria from the turbot GI tract. The large majority of the colonies (82%) were gamma-Proteobacteria, whereas 15.6% and 2.4% of colonies were Firmicutes and Actinobacteria, respectively. At the genus level, 49.4% of all colonies were assigned to Vibrio. Other potential pathogens, including Pseudomonas, Photobacterium, and Enterobacter, and potential probiotics, including Bacillus, Paenibacillus, and Pseudomonas, were also isolated on agar media. Most cultured bacteria belonged to species that were first described in the turbot GI tract. The impact of these species on turbot physiology and health should be investigated further.
关键词	Turbot Gastrointestinal Supernatant 16s Rrna Gene Operational Taxonomic Units (Otus) Cultivation Efficiency
学科领域	Marine & Freshwater Biology ; Oceanography
DOI	10.1007/s00343-014-3099-1
收录类别	SCI
语种	英语
WOS记录号	WOS:000332739600007
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24110
专题	实验海洋生物学重点实验室
通讯作者	Liu, B (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Xing Mengxin,Hou Zhanhui,Qu Yanmei,et al. Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):316-325.
APA	Xing Mengxin,Hou Zhanhui,Qu Yanmei,Liu Bin,&Liu, B .(2014).Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),316-325.
MLA	Xing Mengxin,et al."Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):316-325.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Enhancing the cultur（399KB）			限制开放	ODC PDDL	浏览