Edwardsiella tarda Hfq: impact on host infection and global protein expression

doi:10.1186/1297-9716-45-23

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	Edwardsiella tarda Hfq: impact on host infection and global protein expression
	Hu, Yong-hua 1; Li, Yong-xin 1,2; Sun, Li 1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
	2014-02-25
发表期刊	VETERINARY RESEARCH
卷号	45 页码:23
文章类型	Article
摘要	Hfq is an RNA-binding protein that plays an important role in many cellular processes. In this study, we examined the biological effect of the Hfq of Edwardsiella tarda, a severe fish pathogen with a broad host range that includes humans. To facilitate the study, a markerless hfq in-frame deletion wild type, TXhfq, was constructed. Compared to the wild type TX01, TXhfq exhibited (i) retarded planktonic and biofilm growth, (ii) decreased resistance against oxidative stress, (iii) attenuated overall virulence and tissue dissemination and colonization capacity, (iv) impaired ability to replicate in host macrophages and to block host immune response. Introduction of a trans-expressed hfq gene into TXhfq restored the lost virulence of TXhfq. To identify potential Hfq targets, comparative global proteomic analysis was conducted, which revealed that 20 proteins belonging to different functional categories were differentially expressed in TXhfq and TX01. Quantitative real time RT-PCR analysis showed that the mRNA levels of two thirds of the genes of the identified proteins were consistent with the proteomic results. Since TXhfq is dramatically attenuated in virulence, we further examined its potential as a naturally delivered vaccine administered via the immersion route in a flounder model. The results showed that TXhfq induced effective protection against lethal E. tarda challenge. Taken together, our study indicated that Hfq is required for the normal operation of E. tarda in multiple aspects, and that Hfq probably exerts a regulatory effect on a wide range of target genes at both transcription and post-transcription levels.
学科领域	Veterinary Sciences
DOI	10.1186/1297-9716-45-23
收录类别	SCI
语种	英语
WOS记录号	WOS:000334611700001
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24107
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Taishan Vocat Coll Nursing, Tai An 271000, Shandong, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Hu, Yong-hua,Li, Yong-xin,Sun, Li,et al. Edwardsiella tarda Hfq: impact on host infection and global protein expression[J]. VETERINARY RESEARCH,2014,45:23.
APA	Hu, Yong-hua,Li, Yong-xin,Sun, Li,&Sun, L .(2014).Edwardsiella tarda Hfq: impact on host infection and global protein expression.VETERINARY RESEARCH,45,23.
MLA	Hu, Yong-hua,et al."Edwardsiella tarda Hfq: impact on host infection and global protein expression".VETERINARY RESEARCH 45(2014):23.

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