Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus

doi:10.1093/dnares/dsu025

IOCAS-IR > 实验海洋生物学重点实验室

	Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus
	Gao, Yang1,2 ; Gao, Qiang 1; Zhang, Huan 1; Wang, Lingling 1; Zhang, Fuchong 3; Yang, Chuanyan 1; Song, Linsheng 1; Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China.
	2014-12-01
发表期刊	DNA RESEARCH
卷号	21 期号:6 页码:627-637
文章类型	Article
摘要	The pufferfish Takifugu flavidus is an important economic species due to its outstanding flavour and high market value. It has been regarded as an excellent model of genetic study for decades as well. In the present study, three mate-pair libraries of T. flavidus genome were sequenced by the SOLiD 4 next-generation sequencing platform, and the draft genome was constructed with the short reads using an assisted assembly strategy. The draft consists of 50,947 scaffolds with an N50 value of 305.7 kb, and the average GC content was 45.2%. The combined length of repetitive sequences was 26.5 Mb, which accounted for 6.87% of the genome, indicating that the compactness of T. flavidus genome was approximative with that of T. rubripes genome. A total of 1,253 non-coding RNA genes and 30,285 protein-encoding genes were assigned to the genome. There were 132,775 and 394 presumptive genes playing roles in the colour pattern variation, the relatively slow growth and the lipid metabolism, respectively. Among them, genes involved in the microtubule-dependent transport system, angiogenesis, decapentaplegic pathway and lipid mobilization were significantly expanded in the T. flavidus genome. This draft genome provides a valuable resource for understanding and improving both fundamental and applied research with pufferfish in the future.
关键词	Takifugu Flavidus Draft Genome Ngs
学科领域	Genetics & Heredity
DOI	10.1093/dnares/dsu025
收录类别	SCI
语种	英语
WOS记录号	WOS:000347101100005
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24106
专题	实验海洋生物学重点实验室海洋环境腐蚀与生物污损重点实验室
通讯作者	Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean & Fishery Sci Res Inst Hebei Prov, Qinhuangdao 066200, Hebei, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gao, Yang,Gao, Qiang,Zhang, Huan,et al. Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus[J]. DNA RESEARCH,2014,21(6):627-637.
APA	Gao, Yang.,Gao, Qiang.,Zhang, Huan.,Wang, Lingling.,Zhang, Fuchong.,...&Song, LS .(2014).Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus.DNA RESEARCH,21(6),627-637.
MLA	Gao, Yang,et al."Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus".DNA RESEARCH 21.6(2014):627-637.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Draft Sequencing and（562KB）			限制开放	ODC PDDL	浏览