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Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production
Gao, Yongqiang1,2; Liu, Changshui1,2; Ding, Yamei3; Sun, Chao1; Zhang, Rubing1; Xian, Mo1,4; Zhao, Guang1,4; Xian, M (reprint author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China.
2014-05-16
Source PublicationPLOS ONE
Volume9Issue:5Pages:e97845
SubtypeArticle
AbstractPoly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In our previous study, a pathway for P3HP production was constructed in recombinant Esecherichia coli. Seven exogenous genes in P3HP synthesis pathway were carried by two plasmid vectors. However, the P3HP production was severely suppressed by strain instability due to plasmid loss. In this paper, two strategies, chromosomal gene integration and plasmid addiction system (PAS) based on amino acid anabolism, were applied to construct a genetically stable strain. Finally, a combination of those two methods resulted in the best results. The resultant strain carried a portion of P3HP synthesis genes on chromosome and the others on plasmid, and also brought a tyrosine-auxotrophy based PAS. In aerobic fed-batch fermentation, this strain produced 25.7 g/L P3HP from glycerol, about 2.5-time higher than the previous strain with two plasmids. To the best of our knowledge, this is the highest P3HP production from inexpensive carbon sources.
Subject AreaScience & Technology - Other Topics
DOI10.1371/journal.pone.0097845
Indexed BySCI
Language英语
WOS IDWOS:000339614800076
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/24102
Collection实验海洋生物学重点实验室
Corresponding AuthorXian, M (reprint author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China.
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
4.Collaborat Innovat Ctr Marine Biomass Fibers Mat, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Gao, Yongqiang,Liu, Changshui,Ding, Yamei,et al. Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production[J]. PLOS ONE,2014,9(5):e97845.
APA Gao, Yongqiang.,Liu, Changshui.,Ding, Yamei.,Sun, Chao.,Zhang, Rubing.,...&Xian, M .(2014).Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production.PLOS ONE,9(5),e97845.
MLA Gao, Yongqiang,et al."Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production".PLOS ONE 9.5(2014):e97845.
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