Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas | |
Liu Gang1,2 ; Huan Pin1; Liu Baozhong1; Liu, BZ (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
| |
| 2014-11-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 卷号 | 32期号:6页码:1224-1231 |
| 文章类型 | Article |
| 摘要 | Increasing evidence indicates that transforming growth factor beta (TGF-beta) signaling pathways play many important roles in the early development of mollusks. However, limited information is known concerning their detailed mechanisms. Here, we describe the identification, cloning and characterization of two Smad genes, the key components of TGF-beta signaling pathways, from the Pacific oyster Crassostrea gigas. Sequence analysis of the two genes, designated as cgi-smad1/5/8 and cgi-smad4, revealed conserved functional characteristics. The two genes were widely expressed in embryos and larvae, suggesting multiple roles in the early development of C. gigas. The mRNA of the two genes aggregated in the D quadrant and cgi-smad4 was highly expressed on the dorsal side of the gastrula, indicating that TGF-beta signaling pathways may be involved in dorsoventral patterning in C. gigas. Furthermore, high expression levels of the two genes in the shell fields of embryos at different stages suggested important roles for TGF-beta signaling pathways in particular phases of shell development, including the formation of the initial shell field and the biomineralization of larval shells. The results of this study provide fundamental support for elucidating how TGF-beta signaling pathways participate in the early development of bivalve mollusks, and suggest that further work is warranted to this end. |
| 关键词 | Calcification Mollusk Shell Field Whole Mount In Situ Hybridization Smad Transforming Growth Factor Beta (tgf-Beta) |
| 学科领域 | Marine & Freshwater Biology ; Oceanography |
| DOI | 10.1007/s00343-014-3360-7 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344541900002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24089 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, BZ (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu Gang,Huan Pin,Liu Baozhong,et al. Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(6):1224-1231. |
| APA | Liu Gang,Huan Pin,Liu Baozhong,&Liu, BZ .(2014).Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(6),1224-1231. |
| MLA | Liu Gang,et al."Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.6(2014):1224-1231. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Cloning and expressi（924KB） | 限制开放 | ODC PDDL | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论