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Characterization of Mannitol-2-Dehydrogenase in Saccharina japonica: Evidence for a New Polyol-Specific Long-Chain Dehydrogenases/Reductase
Shao, Zhanru1,2; Zhang, Pengyan1,2; Li, Qiuying1,2; Wang, Xiuliang1; Duan, Delin1; Duan, DL (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
2014-05-15
发表期刊PLOS ONE
卷号9期号:5页码:e97935
文章类型Article
摘要Mannitol plays a crucial role in brown algae, acting as carbon storage, organic osmolytes and antioxidant. Transcriptomic analysis of Saccharina japonica revealed that the relative genes involved in the mannitol cycle are existent. Full-length sequence of mannitol-2-dehydrogenase (M2DH) gene was obtained, with one open reading frame of 2,007 bp which encodes 668 amino acids. Cis-regulatory elements for response to methyl jasmonic acid, light and drought existed in the 5'-upstream region. Phylogenetic analysis indicated that SjM2DH has an ancient prokaryotic origin, and is probably acquired by horizontal gene transfer event. Multiple alignment and spatial structure prediction displayed a series of conserved functional residues, motifs and domains, which favored that SjM2DH belongs to the polyol-specific long-chain dehydrogenases/reductase (PSLDR) family. Expressional profiles of SjM2DH in the juvenile sporophytes showed that it was influenced by saline, oxidative and desiccative factors. SjM2DH was over-expressed in Escherichia coli, and the cell-free extracts with recombinant SjM2DH displayed high activity on D-fructose reduction reaction. The analysis on SjM2DH gene structure and biochemical parameters reached a consensus that activity of SjM2DH is NADH-dependent and metal ion-independent. The characterization of SjM2DH showed that M2DH is a new member of PSLDR family and play an important role in mannitol metabolism in S. japonica.
学科领域Science & Technology - Other Topics
DOI10.1371/journal.pone.0097935
收录类别SCI
语种英语
WOS记录号WOS:000336789500133
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/24083
专题实验海洋生物学重点实验室
通讯作者Duan, DL (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位中国科学院海洋研究所
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GB/T 7714
Shao, Zhanru,Zhang, Pengyan,Li, Qiuying,et al. Characterization of Mannitol-2-Dehydrogenase in Saccharina japonica: Evidence for a New Polyol-Specific Long-Chain Dehydrogenases/Reductase[J]. PLOS ONE,2014,9(5):e97935.
APA Shao, Zhanru,Zhang, Pengyan,Li, Qiuying,Wang, Xiuliang,Duan, Delin,&Duan, DL .(2014).Characterization of Mannitol-2-Dehydrogenase in Saccharina japonica: Evidence for a New Polyol-Specific Long-Chain Dehydrogenases/Reductase.PLOS ONE,9(5),e97935.
MLA Shao, Zhanru,et al."Characterization of Mannitol-2-Dehydrogenase in Saccharina japonica: Evidence for a New Polyol-Specific Long-Chain Dehydrogenases/Reductase".PLOS ONE 9.5(2014):e97935.
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