Biological identification and determination of optimum growth conditions for four species of Navicula

doi:10.1007/s13131-014-0465-y

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	Biological identification and determination of optimum growth conditions for four species of Navicula
	Zhao Xiaobo 1,2; Pang Shaojun 1; Liu Feng 1; Shan Tifeng 1; Li Jing1,2 ; Pang, SJ (reprint author), Chinese Acad Sci, Algal Culture Collect Ctr, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China.
	2014-08-01
发表期刊	ACTA OCEANOLOGICA SINICA
卷号	33 期号:8 页码:111-118
文章类型	Article
摘要	Four species in the genus Navicula were isolated using the serial dilution method. Based on scanning electron microscopy (SEM) and sequence comparisons of two segments of genes (small ribosomal subunit and large subunit of Rubisco), the species were identified as Navicula perminuta, N. pseudacceptata, N. vara, and N. rhynchocephala. Based on phylogenetic analysis and culture trials, there was a close relationship between N. perminuta and N. vara. Growth of these species was evaluated using measurements of optical density at 680 nm (OD680) under various environmental factors. Results showed that the optimum culture conditions were 25 degrees C, 50-100 mol photons m(-2) s(-1), pH 8.0, and salinities from 25 to 30. However, the favorable salinity for N. perminuta was surprisingly high at 35. Nutrient requirement analysis demonstrated that growth of Navicula depended on the availability of SiO32-. Their relative growth rates (RGR) peaked at the highest tested level (0.25 mmol/L). The optimal concentrations of NO3- and PO43- were 3.6 mmol/L and 0.18 mmol/L, respectively. Culture of these Navicula species for abalone or sea cucumber aquaculture should take these factors into consideration.
关键词	Navicula Ssu Rbcl Culture Conditions
学科领域	Oceanography
DOI	10.1007/s13131-014-0465-y
收录类别	SCI
语种	英语
WOS记录号	WOS:000339318800013
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24078
专题	实验海洋生物学重点实验室
通讯作者	Pang, SJ (reprint author), Chinese Acad Sci, Algal Culture Collect Ctr, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Algal Culture Collect Ctr, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhao Xiaobo,Pang Shaojun,Liu Feng,et al. Biological identification and determination of optimum growth conditions for four species of Navicula[J]. ACTA OCEANOLOGICA SINICA,2014,33(8):111-118.
APA	Zhao Xiaobo,Pang Shaojun,Liu Feng,Shan Tifeng,Li Jing,&Pang, SJ .(2014).Biological identification and determination of optimum growth conditions for four species of Navicula.ACTA OCEANOLOGICA SINICA,33(8),111-118.
MLA	Zhao Xiaobo,et al."Biological identification and determination of optimum growth conditions for four species of Navicula".ACTA OCEANOLOGICA SINICA 33.8(2014):111-118.

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